Absence of nuclease activity in commonly used oxygen-scavenging systems
File(s)2017 Gahlon BMR Research Notes.pdf (1.21 MB)
Published version
Author(s)
Gahlon, HL
Poudevigne-Durance, P
Rueda, D
Type
Journal Article
Abstract
Objective
Oxygen scavenging systems are routinely used during single-molecule imaging experiments to improve fluorescent dye stability. Previous work has shown nuclease contamination in the commonly used oxygen scavenging systems. This study evaluates the potential for nuclease contamination in these oxygen scavenging systems.
Results
Linear and plasmid DNA was incubated with two different oxygen scavenging systems (1) protocatechuic acid (PCA)-protocatechuate-3,4-dioxygenase (PCD) and (2) glucose-coupled glucose oxidase/catalase (GODCAT). No nucleic acid degradation was observed on single and double-stranded linear DNA and plasmid DNA, indicating the absence of nuclease contamination in these oxygen scavenging systems.
Oxygen scavenging systems are routinely used during single-molecule imaging experiments to improve fluorescent dye stability. Previous work has shown nuclease contamination in the commonly used oxygen scavenging systems. This study evaluates the potential for nuclease contamination in these oxygen scavenging systems.
Results
Linear and plasmid DNA was incubated with two different oxygen scavenging systems (1) protocatechuic acid (PCA)-protocatechuate-3,4-dioxygenase (PCD) and (2) glucose-coupled glucose oxidase/catalase (GODCAT). No nucleic acid degradation was observed on single and double-stranded linear DNA and plasmid DNA, indicating the absence of nuclease contamination in these oxygen scavenging systems.
Date Issued
2017-11-21
Date Acceptance
2017-11-14
Citation
BMC Research Notes, 2017, 10
ISSN
1756-0500
Publisher
BioMed Central
Journal / Book Title
BMC Research Notes
Volume
10
Copyright Statement
© The Author(s) 2017. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License
(
http://creativecommons.org/licenses/by/4.0/
), which permits unrestricted use, distribution, and reproduction in any medium,
provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license,
and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (
http://creativecommons.org/
publicdomain/zero/1.0/
) applies to the data made available in this article, unless otherwise stated.
(
http://creativecommons.org/licenses/by/4.0/
), which permits unrestricted use, distribution, and reproduction in any medium,
provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license,
and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (
http://creativecommons.org/
publicdomain/zero/1.0/
) applies to the data made available in this article, unless otherwise stated.
Sponsor
Commission of the European Communities
Marie Skłodowska Curie Actions
Medical Research Council
Grant Number
705015
705015
MC_UP_1102/5
Subjects
Catalase
Glucose oxidase
Oxygen scavenging system
Protocatechuate-3,4-dioxygenase
Protocatechuic acid
Single-molecule imaging
1199 Other Medical And Health Sciences
Bioinformatics
Publication Status
Published
Article Number
606
Date Publish Online
2017-11-21