One-pot synthesis of multiple protein-encapsulated DNA flowers and their application in intracellular protein delivery
File(s)
Published version
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Author(s)
Type
Journal Article
Abstract
Inspired by biological systems, many biomimetic methods suggest fabrication of functional materials with unique physicochemical properties. Such methods frequently generate organic–inorganic composites that feature highly ordered hierarchical structures with intriguing properties, distinct from their individual components. A striking example is that of DNA–inorganic hybrid micro/nanostructures, fabricated by the rolling circle technique. Here, a novel concept for the encapsulation of bioactive proteins in DNA flowers (DNF) while maintaining the activity of protein payloads is reported. A wide range of proteins, including enzymes, can be simultaneously associated with the growing DNA strands and Mg2PPi crystals during the rolling circle process, ultimately leading to the direct immobilization of proteins into DNF. The unique porous structure of this construct, along with the abundance of Mg ions and DNA molecules present, provides many interaction sites for proteins, enabling high loading efficiency and enhanced stability. Further, as a proof of concept, it is demonstrated that the DNF can deliver payloads of cytotoxic protein (i.e., RNase A) to the cells without a loss in its biological function and structural integrity, resulting in highly increased cell death compared to the free protein.
Date Issued
2017-05-05
Date Acceptance
2017-03-31
Citation
Advanced Materials, 2017, 29 (26)
ISSN
1521-4095
Publisher
Wiley
Journal / Book Title
Advanced Materials
Volume
29
Issue
26
Copyright Statement
© 2017 The Authors. Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
License URL
Sponsor
British Heart Foundation
Engineering & Physical Science Research Council (EPSRC)
Commission of the European Communities
Commission of the European Communities
Engineering and Physical Sciences Research Council
Grant Number
RE/08/002/23906
EP/K020641/1
ERC-2013-CoG-616417
659175
EP/L015498/1
Subjects
Science & Technology
Physical Sciences
Technology
Chemistry, Multidisciplinary
Chemistry, Physical
Nanoscience & Nanotechnology
Materials Science, Multidisciplinary
Physics, Applied
Physics, Condensed Matter
Chemistry
Science & Technology - Other Topics
Materials Science
Physics
cytotoxicity
DNA flowers
one-pot synthesis
protein delivery
protein encapsulation
ROLLING-CIRCLE AMPLIFICATION
BOVINE SERUM-ALBUMIN
DRUG-DELIVERY
BIOMEDICAL APPLICATIONS
EFFICIENT DELIVERY
SIRNA DELIVERY
CANCER-THERAPY
NANOSTRUCTURES
ORIGAMI
NANOPARTICLES
02 Physical Sciences
03 Chemical Sciences
09 Engineering
Publication Status
Published
Article Number
1701086