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Sgs1’s roles in DNA end resection, HJ
dissolution, and crossover suppression
require a two-step SUMO regulation
dependent on Smc5/6
File(s)
Author(s)
Bermudez-Lopez, M
Villoria, MT
Esteras, M
Jarmuz, A
Torres-Rosell, J
Type
Journal Article
Abstract
The RecQ helicase Sgs1 plays critical roles during DNA repair by homologous recombination, from end resection to Holliday junction (HJ) dissolution. Sgs1 has both pro- and anti-recombinogenic roles, and therefore its activity must be tightly regulated. However, the controls involved in recruitment and activation of Sgs1 at damaged sites are unknown. Here we show a two-step role for Smc5/6 in recruiting and activating Sgs1 through SUMOylation. First, auto-SUMOylation of Smc5/6 subunits leads to recruitment of Sgs1 as part of the STR (Sgs1–Top3–Rmi1) complex, mediated by two SUMO-interacting motifs (SIMs) on Sgs1 that specifically recognize SUMOylated Smc5/6. Second, Smc5/6-dependent SUMOylation of Sgs1 and Top3 is required for the efficient function of STR. Sgs1 mutants impaired in recognition of SUMOylated Smc5/6 (sgs1-SIMΔ) or SUMO-dead alleles (sgs1-KR) exhibit unprocessed HJs at damaged replication forks, increased crossover frequencies during double-strand break repair, and severe impairment in DNA end resection. Smc5/6 is a key regulator of Sgs1's recombination functions.
Date Issued
2016-06-01
Date Acceptance
2016-05-09
Citation
Genes & Development, 2016, 30, pp.1339-1356
URI
URL
DOI
ISSN
1549-5477
Publisher
Cold Spring Harbor Laboratory Press
Start Page
1339
End Page
1356
Journal / Book Title
Genes & Development
Volume
30
Copyright Statement
© 2016 Bermúdez-López et al. This article, published in Genes & Development,
is available under a CreativeCommons License (Attribution 4.0 International),
as described at http://creativecommons.org/licenses/by/4.0/.
License URL
Sponsor
Wellcome Trust
Grant Number
100955/Z/13/Z
Subjects
Developmental Biology
06 Biological Sciences
11 Medical And Health Sciences
17 Psychology And Cognitive Sciences
Publication Status
Published
Date Publish Online
2016-06-01