Synthetic biology and bioelectrochemical tools for electrogenetic system engineering
File(s)
Author(s)
Ledesma Amaro, Rodrigo
Ouldridge, thomas
O'Hare, Danny
Team, iGEM
Baldwin, Geoff
Type
Journal Article
Abstract
Synthetic biology research and its industrial applications rely on deterministic spatiotemporal control of gene expression. Recently, electrochemical control of gene expression has been demonstrated in electrogenetic systems (redox-responsive promoters used alongside redox inducers and electrodes), allowing for the direct integration of electronics with biological processes. However, use of electrogenetic systems is limited by poor activity, tunability and standardisation. In this work we developed a strong, unidirectional, redox-responsive promoter before deriving a mutant promoter library with a spectrum of strengths. We constructed genetic circuits with these parts and demonstrated their activation by multiple classes of redox molecules. Finally, we demonstrated electrochemical activation of gene expression in aerobic conditions using a novel, modular bioelectrochemical device. These genetic and electrochemical tools facilitate the design and improve the performance of electrogenetic systems. Furthermore, the genetic design strategies used can be applied to other redox-responsive promoters to further expand the available tools for electrogenetics.
Date Acceptance
2022-03-02
Citation
Science Advances, 8 (18)
ISSN
2375-2548
Publisher
American Association for the Advancement of Science
Journal / Book Title
Science Advances
Volume
8
Issue
18
Copyright Statement
Copyright © 2022
The Authors, some
rights reserved;
exclusive licensee
American Association
for the Advancement
of Science. No claim to
original U.S.Government
Works. Distributed
under a Creative
Commons Attribution
License 4.0 (CC BY)
The Authors, some
rights reserved;
exclusive licensee
American Association
for the Advancement
of Science. No claim to
original U.S.Government
Works. Distributed
under a Creative
Commons Attribution
License 4.0 (CC BY)
License URL
Sponsor
Biotechnology and Biological Sciences Research Council (BBSRC)
Biotechnology and Biological Sciences Research Council (BBSRC)
The Royal Society
Biotechnology and Biological Sciences Research Council (BBSRC)
British Council (UK)
Commission of the European Communities
Commission of the European Communities
Identifier
https://www.science.org/doi/10.1126/sciadv.abm5091
Grant Number
BB/R01602X/1
BB/T011408/1
NAF\R1\201187
BB/T013176/1
527429894
949080
101022370
Subjects
PixCell iGEM Team
Publication Status
Published