Metabolic engineering of Rhodotorula toruloides for the production of linalool
Author(s)
Type
Journal Article
Abstract
Linalool is widely used in foods, pharmaceuticals, and cosmetics. Microbial production offers a sustainable alternative to current plant extraction and chemical synthesis. In the present study, the oleaginous yeast Rhodotorula toruloides was metabolically engineered for linalool production. Overall, an integrated strategy involving linalool synthase selection, promoter screening, and pathway enhancement was implemented. The linalool biosynthesis baseline was established by using a 51 aa-truncated linalool synthase from Mentha citrate (t51McLIS). The linalool titer was increased to 249.7 mg/L by optimizing the expression of critical enzymes with native promoters. Then, it was improved to 790.2 mg/L by manipulating the mevalonate pathway and further elevated to 845.1 mg/L (42.3 mg/g glucose) by optimizing the fermentation conditions in shake flasks. Finally, the linalool production was enhanced to 2.59 g/L and 26.9 mg/g glucose in a 3 L bioreactor. The results demonstrated the potential of R. toruloides for the microbial production of monoterpenes.
Date Issued
2025-03-05
Date Acceptance
2025-02-14
Citation
Journal of Agricultural and Food Chemistry, 2025, 73 (9), pp.5395-5404
ISSN
0021-8561
Publisher
American Chemical Society
Start Page
5395
End Page
5404
Journal / Book Title
Journal of Agricultural and Food Chemistry
Volume
73
Issue
9
Copyright Statement
Copyright © 2025 American Chemical Society. This is the author’s accepted manuscript made available under a CC-BY licence in accordance with Imperial’s Research Publications Open Access policy (www.imperial.ac.uk/oa-policy)
License URL
Identifier
https://www.ncbi.nlm.nih.gov/pubmed/39971727
Subjects
linalool
metabolic engineering
microbial production
monoterpene
synthetic biology
Publication Status
Published
Coverage Spatial
United States
Date Publish Online
2025-02-19