Ultrastructure and complex polar architecture of the human pathogen Campylobacter jejuni
Author(s)
Type
Journal Article
Abstract
Campylobacter jejuni is one of the most successful food-borne human
pathogens. Here we use electron cryotomography to explore the ultrastructure
of C. jejuni cells in logarithmically growing cultures. This provides the first look
at this pathogen in a near-native state at macromolecular resolution (~5 nm).
We find a surprisingly complex polar architecture that includes ribosome
exclusion zones, polyphosphate storage granules, extensive collar-shaped
chemoreceptor arrays, and elaborate flagellar motors.
pathogens. Here we use electron cryotomography to explore the ultrastructure
of C. jejuni cells in logarithmically growing cultures. This provides the first look
at this pathogen in a near-native state at macromolecular resolution (~5 nm).
We find a surprisingly complex polar architecture that includes ribosome
exclusion zones, polyphosphate storage granules, extensive collar-shaped
chemoreceptor arrays, and elaborate flagellar motors.
Date Issued
2014-07-25
Date Acceptance
2014-06-27
Citation
MicrobiologyOpen, 2014, 3 (5), pp.702-710
ISSN
2045-8827
Publisher
Wiley
Start Page
702
End Page
710
Journal / Book Title
MicrobiologyOpen
Volume
3
Issue
5
Copyright Statement
© 2014 The Authors. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Subjects
Science & Technology
Life Sciences & Biomedicine
Microbiology
Acidocalcisomes
Campylobacterales
chemoreceptors
cryoelectron tomography
cryo-EM
food poisoning
polyphosphate storage granules
GUILLAIN-BARRE-SYNDROME
ESCHERICHIA-COLI
ELECTRON-MICROSCOPY
INORGANIC POLYPHOSPHATE
PSEUDOMONAS-AERUGINOSA
BACTERIA
LOCALIZATION
TOMOGRAPHY
INFECTION
CELLS
Campylobacter Infections
Campylobacter jejuni
Cell Polarity
Cryoelectron Microscopy
Humans
Organelles
Publication Status
Published