Aureochrome 1 illuminated: structural changes of a transcription factor probed by molecular spectroscopy
Author(s)
Kerruth, Silke
Ataka, Kenichi
Frey, Daniel
Schlichting, Ilme
Heberle, Joachim
Type
Journal Article
Abstract
Aureochrome 1 from Vaucheria frigida is a recently identified blue-light receptor that acts as a transcription factor. The protein comprises a photosensitive light-, oxygen- and voltage-sensitive (LOV) domain and a basic zipper (bZIP) domain that binds DNA rendering aureochrome 1 a prospective optogenetic tool. Here, we studied the photoreaction of full-length aureochrome 1 by molecular spectroscopy. The kinetics of the decay of the red-shifted triplet state and the blue-shifted signaling state were determined by time-resolved UV/Vis spectroscopy. It is shown that the presence of the bZIP domain further prolongs the lifetime of the LOV390 signaling state in comparison to the isolated LOV domain whereas bound DNA does not influence the photocycle kinetics. The light-dark Fourier transform infrared (FTIR) difference spectrum shows the characteristic features of the flavin mononucleotide chromophore except that the S-H stretching vibration of cysteine 254, which is involved in the formation of the thio-adduct state, is significantly shifted to lower frequencies compared to other LOV domains. The presence of the target DNA influences the light-induced FTIR difference spectrum of aureochrome 1. Vibrational bands that can be assigned to arginine and lysine side chains as well to the phosphate backbone, indicate crucial changes in interactions between transcription factor and DNA.
Date Issued
2014-07-24
Date Acceptance
2014-06-29
Citation
PLoS One, 2014, 9 (7), pp.1-8
ISSN
1932-6203
Publisher
Public Library of Science (PLoS)
Start Page
1
End Page
8
Journal / Book Title
PLoS One
Volume
9
Issue
7
Copyright Statement
© 2014 Kerruth et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Identifier
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000341354800081&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
Subjects
Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
BLUE-LIGHT RECEPTOR
LOV DOMAIN
CONFORMATIONAL-CHANGES
BACILLUS-SUBTILIS
PHOTORECEPTOR PHOTOTROPIN
CHLAMYDOMONAS-REINHARDTII
CRYSTAL-STRUCTURES
SIGNALING PATHWAY
PHOT-LOV1 DOMAIN
BINDING
Publication Status
Published
Article Number
ARTN e103307
Date Publish Online
2014-07-24