Flow dynamics and HSPC homing in bone marrow microvessels.
File(s)
Published version
Author(s)
Type
Journal Article
Abstract
Measurements of flow velocities at the level of individual arterial vessels and sinusoidal capillaries are crucial for understanding the dynamics of hematopoietic stem and progenitor cell homing in the bone marrow vasculature. We have developed two complementary intravital two-photon imaging approaches to determine blood flow dynamics and velocities in multiple vessel segments by capturing the motion of red blood cells. High-resolution spatiotemporal measurements through a cranial window to determine short-time dynamics of flowing blood cells and repetitive centerline scans were used to obtain a detailed flow-profile map with hemodynamic parameters. In addition, we observed the homing of individual hematopoietic stem and progenitor cells and obtained detailed information on their homing behavior. With our imaging setup, we determined flow patterns at cellular resolution, blood flow velocities and wall shear stress in small arterial vessels and highly branched sinusoidal capillaries, and the cellular dynamics of hematopoietic stem and progenitor cell homing.
Date Issued
2017-02-14
Date Acceptance
2017-01-18
Citation
Cell Reports, 2017, 18 (7), pp.1804-1816
ISSN
2211-1247
Publisher
Elsevier
Start Page
1804
End Page
1816
Journal / Book Title
Cell Reports
Volume
18
Issue
7
Copyright Statement
© 2017 The Author(s). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Identifier
http://www.ncbi.nlm.nih.gov/pubmed/28199850
PII: S2211-1247(17)30097-9
Subjects
blood flow velocities
bone marrow
deep tissue imaging
hematopoietic parameters
hematopoietic stem cell
intravital imaging
microvasculature
stem cell homing
wall shear stress
Publication Status
Published
Coverage Spatial
United States