In situ electron microscopy of complex biological and nanoscale systems: challenges and opportunities
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Published version
Author(s)
Han, Zexiang
Porter, Alexandra E
Type
Journal Article
Abstract
In situ imaging for direct visualization is important for physical and biological sciences. Research endeavors into elucidating dynamic biological and nanoscale phenomena frequently necessitate in situ and time-resolved imaging. In situ liquid cell electron microscopy (LC-EM) can overcome certain limitations of conventional electron microscopies and offer great promise. This review aims to examine the status-quo and practical challenges of in situ LC-EM and its applications, and to offer insights into a novel correlative technique termed microfluidic liquid cell electron microscopy. We conclude by suggesting a few research ideas adopting microfluidic LC-EM for in situ imaging of biological and nanoscale systems.
Date Issued
2020-12-03
Date Acceptance
2020-11-13
Citation
Frontiers in Nanotechnology, 2020, 2, pp.1-14
ISSN
2673-3013
Publisher
Frontiers Media
Start Page
1
End Page
14
Journal / Book Title
Frontiers in Nanotechnology
Volume
2
Copyright Statement
© 2020 Han and Porter. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
License URL
Identifier
https://www.frontiersin.org/articles/10.3389/fnano.2020.606253/full
Publication Status
Published
Date Publish Online
2020-12-03