A multiplexed targeted assay for high-throughput quantitative analysis of serum methylamines by ultra performance liquid chromatography coupled to high resolution mass spectrometry
File(s)
Accepted version
Author(s)
Type
Journal Article
Abstract
Methylamines are biologically-active metabolites present in serum and urine samples, which play complex roles in metabolic diseases. Methylamines can be detected by proton nuclear magnetic resonance (NMR), but specific methods remain to be developed for their routine assay in human serum in clinical settings. Here we developed and validated a novel reliable “methylamine panel” method for simultaneous quantitative analysis of trimethylamine (TMA), its major detoxification metabolite trimethylamine-N-oxide (TMAO), and precursors choline, betaine and l-carnitine in human serum using Ultra Performance Liquid Chromatography (UPLC) coupled to High Resolution Mass Spectrometry (HRMS). Metabolite separation was carried out on a HILIC stationary phase. For all metabolites, the assay was linear in the range of 0.25–12.5 μmol/L and enabled to reach limit of detection of about 0.10 μmol/L. Relative standard deviations were below 16% for the three levels of concentrations. We demonstrated the strong reliability and robustness of the method, which was applied to serum samples from healthy individuals to establish the range of concentrations of the metabolites and their correlation relationships and detect gender differences. Our data provide original information for implementing in a clinical environment a MS-based diagnostic method with potential for targeted metabolic screening of patients at risk of cardiometabolic diseases.
Date Issued
2016-03-30
Date Acceptance
2016-03-28
Citation
Archives of Biochemistry and Biophysics, 2016, 597, pp.12-20
ISSN
1096-0384
Publisher
Elsevier
Start Page
12
End Page
20
Journal / Book Title
Archives of Biochemistry and Biophysics
Volume
597
Copyright Statement
© 2016 Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/
Subjects
Science & Technology
Life Sciences & Biomedicine
Biochemistry & Molecular Biology
Biophysics
Trimethylamine
Trimethylamine-N-oxide
Choline
Betaine
L-carnitine
Cardiometabolic diseases
TRIMETHYLAMINE-N-OXIDE
FISH-ODOR SYNDROME
ELECTROSPRAY-IONIZATION
URINARY TRIMETHYLAMINE
L-CARNITINE
METABOLISM
MICROBIOTA
PLASMA
DISEASE
CHOLINE
l-carnitine
0601 Biochemistry And Cell Biology
Publication Status
Published