Contact lens sensor for ocular inflammation monitoring
File(s)
Published version
Author(s)
Type
Journal Article
Abstract
Contact lens sensors have been emerging as point-of-care devices in recent healthcare developments for ocular physiological condition monitoring and diagnosis. Fluorescence sensing technologies have been widely applied in contact lens sensors due to their accuracy, high sensitivity, and specificity. As ascorbic acid (AA) level in tears is closely related to ocular inflammation, a fluorescent contact lens sensor incorporating a BSA-Au nanocluster (NC) probe is developed for in situ tear AA detection. The NCs are firstly synthesized to obtain a fluorescent probe, which exhibits high reusability through the quench/recover (KMnO4/AA) process. The probe is then encapsulated with 15 wt% of poly(vinyl alcohol) (PVA) and 1.5 wt% of citric acid (CA) film, and implemented on a closed microfluidic contact lens sensing region. The laser-ablated microfluidic channel in contact lens sensors allows for tear fluid to flow through the sensing region, enabling an in-situ detection of AA. Meanwhile, a smartphone application accompanied by a customized 3D printed readout box is developed for image caption and algorism to quantitative analysis of AA levels. The contact lens sensor is tested within the readout box and the emission signal is collected through the smartphone camera at room temperature with an achieved LOD of 0.178 mmol L-1 (0.0–1.2 mmol L-1). The operational and storage lifetime is also evaluated to characterize the sensor properties and resulted in 20 h and 10 days, respectively. The reusable AA contact lens sensor is promising to lead to an alternative accessible diagnostic method for ocular inflammation in point-of-care settings.
Date Issued
2024-04-01
Date Acceptance
2024-01-03
Citation
Biosensors and Bioelectronics, 2024, 249
ISSN
0956-5663
Publisher
Elsevier
Journal / Book Title
Biosensors and Bioelectronics
Volume
249
Copyright Statement
© 2024 The Author(s). Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
License URL
Identifier
https://www.ncbi.nlm.nih.gov/pubmed/38227993
PII: S0956-5663(24)00006-X
Subjects
Ascorbic acid
ASCORBIC-ACID
Biophysics
Biotechnology & Applied Microbiology
Chemistry
Chemistry, Analytical
Contact lens sensor
DEVICE
DRY EYE DISEASE
Electrochemistry
Inflammatory dry eye disease
LACTOFERRIN
Life Sciences & Biomedicine
NANOCLUSTERS
Nanoscience & Nanotechnology
Ocular analysis
Physical Sciences
Point -of -care diagnosis
PROBE
REDUCED GRAPHENE OXIDE
Reversible fluorescent sensor
Science & Technology
Science & Technology - Other Topics
Smartphone devices
SURFACE
URIC-ACID
VITAMIN-C
Publication Status
Published
Coverage Spatial
England
Article Number
116003
Date Publish Online
2024-01-06