Pseudomonas putida JM37 as a novel bacterial chassis for ethylene glycol upcycling
File(s)
Published version
Author(s)
Molpeceres-García, Francisco J
García-Miró, Alejandro
Mateos-García, Elvira
Prieto, Alicia
Sanz, David
Type
Journal Article
Abstract
Ethylene glycol (EG), one of the main monomers of polyethylene terephthalate (PET), is an attractive target for microbial upcycling. Despite this interest, there is a limited number of described organisms that can efficiently metabolise EG. Here, we report the metabolic and biotechnological potential of Pseudomonas putida JM37 as a novel bacterial chassis for EG valorization. We show that JM37 efficiently grows on EG as the sole carbon and energy source, outperforming other Pseudomonas strains. Genome sequencing and directed mutagenesis revealed that genetic redundancies in the glyoxylate assimilation pathways underlie its robust EG metabolism. Beyond biomass generation, we demonstrated the biotechnological potential of JM37. This strain was able to accumulate medium-chain polyhydroxyalkanoates (mcl-PHAs), dominated by C10 monomers, directly from EG. Moreover, JM37 successfully expressed heterologous biosynthetic pathways, including a violacein biosynthetic operon and a PET-hydrolase which has been secreted actively into the extracellular medium. Together, our results support the use of P. putida JM37 as a versatile synthetic biology chassis for sustainable EG upcycling and as a promising platform for circular bioproduction.
Date Issued
2026-03-01
Date Acceptance
2025-12-24
Citation
Bioresource Technology, 2026, 443
ISSN
0960-8524
Publisher
Elsevier BV
Journal / Book Title
Bioresource Technology
Volume
443
Copyright Statement
© 2025 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).
License URL
Publication Status
Published
Article Number
133884
Date Publish Online
2025-12-24