Segmental outflow dynamics in the trabecular meshwork of living mice
File(s)
Published version
Author(s)
Reina-Torres, Ester
Baptiste, Tiffany MG
Overby, Darryl R
Type
Journal Article
Abstract
Aqueous humour does not drain uniformly through the trabecular meshwork (TM), but rather follows nonuniform or “segmental” routes. In this study, we examined whether segmental outflow patterns in the TM
change over time in living mice and whether such changes are affected by age. Segmental outflow patterns were
labelled by constant-pressure infusion of fluorescent tracer microparticles into the anterior chamber of anesthetised C57BL/6J mice at 3 or 8 months of age. Two different tracer colours were infused at separate time points
with an interval of Δt = 0, 2, 7 or 14 days. In a separate experiment, one tracer was infused in vivo while the
second tracer was infused ex vivo after 2 days. The spatial relationship between the two tracer patterns was
analysed using the Pearson’s correlation coefficient, r. In 3-month-old mice, there was a time-dependent decay in
r, which was near unity at Δt = 0 and near zero at Δt = 14 days. In 8-month-old mice, r remained elevated for 14
days. Segmental outflow patterns measured in young mice ex vivo were not significantly different from those
measured in vivo after accounting for the expected changes over 2 days. Therefore, segmental outflow patterns
are not static in the TM but redistribute over time, achieving near complete loss of correlation by 2 weeks in
young healthy mice. There is an age-related decline in the rate at which segmental outflow patterns redistribute
in the TM. Further research is needed to understand the dynamic factors controlling segmental outflow.
change over time in living mice and whether such changes are affected by age. Segmental outflow patterns were
labelled by constant-pressure infusion of fluorescent tracer microparticles into the anterior chamber of anesthetised C57BL/6J mice at 3 or 8 months of age. Two different tracer colours were infused at separate time points
with an interval of Δt = 0, 2, 7 or 14 days. In a separate experiment, one tracer was infused in vivo while the
second tracer was infused ex vivo after 2 days. The spatial relationship between the two tracer patterns was
analysed using the Pearson’s correlation coefficient, r. In 3-month-old mice, there was a time-dependent decay in
r, which was near unity at Δt = 0 and near zero at Δt = 14 days. In 8-month-old mice, r remained elevated for 14
days. Segmental outflow patterns measured in young mice ex vivo were not significantly different from those
measured in vivo after accounting for the expected changes over 2 days. Therefore, segmental outflow patterns
are not static in the TM but redistribute over time, achieving near complete loss of correlation by 2 weeks in
young healthy mice. There is an age-related decline in the rate at which segmental outflow patterns redistribute
in the TM. Further research is needed to understand the dynamic factors controlling segmental outflow.
Date Issued
2022-12
Date Acceptance
2022-10-14
Citation
Experimental Eye Research, 2022, 225
ISSN
0014-4835
Publisher
Elsevier
Journal / Book Title
Experimental Eye Research
Volume
225
Copyright Statement
© 2022 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
License URL
Identifier
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000890466300005&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=a2bf6146997ec60c407a63945d4e92bb
Subjects
ANGIOGRAPHY
Aqueous humour outflow
AQUEOUS-HUMOR OUTFLOW
CATIONIC FERRITIN
FLOW
HUMAN EYES
INNER-WALL
Life Sciences & Biomedicine
Mouse models
Ophthalmology
PATTERNS
Science & Technology
Segmental outflow
Trabecular meshwork
Publication Status
Published
Article Number
109285
Date Publish Online
2022-10-21