Insights into the mechanism of action of the arbitrium communication system in SPbeta phages
File(s)s41467-022-31144-3.pdf (3.45 MB)
Published version
Author(s)
Penades, J
Gallego del Sol, F
Quiles-Puchalt, N
Brady, A
Marina, A
Type
Journal Article
Abstract
The arbitrium system is employed by phages of the SPbeta family to communicate with their progeny during infection to decide either to follow the lytic or the lysogenic cycle. The system is controlled by a peptide, AimP, that binds to the regulator AimR, inhibiting its DNA-binding activity and expression of aimX. Although the structure of AimR has been elucidated for phages SPβ and phi3T, there is still controversy regarding the molecular mechanism of AimR function, with two different proposed models for SPβ. In this study, we deepen our understanding of the system by solving the structure of an additional AimR that shows chimerical characteristics with the SPβ receptor. The crystal structures of this AimR (apo, AimP-bound and DNA-bound) together with in vitro and in vivo analyses confirm a mechanism of action by AimP-induced conformational restriction, shedding light on peptide specificity and cross regulation with relevant biological implications.
Date Acceptance
2022-06-01
ISSN
2041-1723
Publisher
Nature Research
Journal / Book Title
Nature Communications
Volume
13
Copyright Statement
Open Access This article is licensed under a Creative Commons
Attribution 4.0 International License, which permits use, sharing,
adaptation, distribution and reproduction in any medium or format, as long as you give
appropriate credit to the original author(s) and the source, provide a link to the Creative
Commons license, and indicate if changes were made. The images or other third party
material in this article are included in the article’s Creative Commons license, unless
indicated otherwise in a credit line to the material. If material is not included in the
article’s Creative Commons license and your intended use is not permitted by statutory
regulation or exceeds the permitted use, you will need to obtain permission directly from
the copyright holder. To view a copy of this license, visit http://creativecommons.org/
licenses/by/4.0/.
© The Author(s) 2022
Attribution 4.0 International License, which permits use, sharing,
adaptation, distribution and reproduction in any medium or format, as long as you give
appropriate credit to the original author(s) and the source, provide a link to the Creative
Commons license, and indicate if changes were made. The images or other third party
material in this article are included in the article’s Creative Commons license, unless
indicated otherwise in a credit line to the material. If material is not included in the
article’s Creative Commons license and your intended use is not permitted by statutory
regulation or exceeds the permitted use, you will need to obtain permission directly from
the copyright holder. To view a copy of this license, visit http://creativecommons.org/
licenses/by/4.0/.
© The Author(s) 2022
License URI
Sponsor
Biotechnology and Biological Sciences Research Council (BBSRC)
Medical Research Council (MRC)
Medical Research Council (MRC)
Biotechnology and Biological Sciences Research Cou
Identifier
https://www.nature.com/articles/s41467-022-31144-3
Grant Number
BB/V002376/1
MR/V000772/1
MR/S00940X/2
BB/V009583/1
Subjects
Bacillus Phages
Bacteriophages
Communication
DNA
Lysogeny
Peptides
Bacteriophages
Bacillus Phages
Peptides
DNA
Communication
Lysogeny
Publication Status
Published