alpha-actinin accounts for the bioactivity of actin preparations in inducing STAT target genes in Drosophila melanogaster
Author(s)
Type
Journal Article
Abstract
Damage-associated molecular patterns (DAMPs) are molecules exposed or released by
dead cells that trigger or modulate immunity and tissue repair. In vertebrates, the cytoskeletal
component F-actin is a DAMP specifically recognised by DNGR-1, an innate immune receptor.
Previously we suggested that actin is also a DAMP in Drosophila melanogaster by inducing STATdependent genes (Srinivasan et al., 2016). Here, we revise that conclusion and report that aactinin is far more potent than actin at inducing the same STAT response and can be found in trace
amounts in actin preparations. Recombinant expression of actin or a-actinin in bacteria
demonstrated that only a-actinin could drive the expression of STAT target genes in Drosophila.
The response to injected a-actinin required the same signalling cascade that we had identified in
our previous work using actin preparations. Taken together, these data indicate that a-actinin
rather than actin drives STAT activation when injected into Drosophila.
dead cells that trigger or modulate immunity and tissue repair. In vertebrates, the cytoskeletal
component F-actin is a DAMP specifically recognised by DNGR-1, an innate immune receptor.
Previously we suggested that actin is also a DAMP in Drosophila melanogaster by inducing STATdependent genes (Srinivasan et al., 2016). Here, we revise that conclusion and report that aactinin is far more potent than actin at inducing the same STAT response and can be found in trace
amounts in actin preparations. Recombinant expression of actin or a-actinin in bacteria
demonstrated that only a-actinin could drive the expression of STAT target genes in Drosophila.
The response to injected a-actinin required the same signalling cascade that we had identified in
our previous work using actin preparations. Taken together, these data indicate that a-actinin
rather than actin drives STAT activation when injected into Drosophila.
Date Issued
2018-09-27
Date Acceptance
2018-09-26
Citation
eLife, 2018, 7
ISSN
2050-084X
Publisher
eLife Sciences Publications Ltd
Journal / Book Title
eLife
Volume
7
Copyright Statement
© 2018 Gordon et al. This
article is distributed under the
terms of the Creative Commons
Attribution License (https://creativecommons.org/licenses/by/4.0/), which
permits unrestricted use and
redistribution provided that the
original author and source are
credited.
article is distributed under the
terms of the Creative Commons
Attribution License (https://creativecommons.org/licenses/by/4.0/), which
permits unrestricted use and
redistribution provided that the
original author and source are
credited.
Identifier
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000446156000001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
Subjects
Science & Technology
Life Sciences & Biomedicine
Biology
Life Sciences & Biomedicine - Other Topics
HUMORAL STRESS-RESPONSE
SEPTIC INJURY
CELL-DEATH
F-ACTIN
RECEPTOR
IMMUNITY
DNGR-1
Publication Status
Published
Article Number
e38636
Date Publish Online
2018-09-27