Clonality of HIV-1 and HTLV-1 infected cells in naturally coinfected individuals
File(s)
Published version
OA Location
Author(s)
Type
Journal Article
Abstract
BACKGROUND: Coinfection with HIV-1 and HTLV-1 diminishes the value of the CD4 + T-cell count in diagnosing AIDS, and increases the rate of HTLV-1-associated myelopathy. It remains elusive how HIV-1/HTLV-1 coinfection is related to such clinical characteristics. Here, we investigated the mutual effect of HIV-1/HTLV-1 coinfection on their integration sites (ISs) and the clonal expansion. METHODS: We extracted DNA from longitudinal peripheral blood samples from 7 HIV-1/HTLV-1 coinfected individuals, and from 12 HIV-1 and 13 HTLV-1 mono-infected individuals. The proviral loads (PVL) were quantified using real-time PCR. Viral ISs and clonality were quantified by ligation-mediated PCR followed by high-throughput sequencing. RESULTS: The PVL of both HIV-1 and HTLV-1 in coinfected individuals was significantly higher than that of the respective virus in mono-infected individuals. The degree of oligoclonality of both HIV-1- and HTLV-1-infected cells in co-infected individuals was also greater than that in mono-infected subjects. The ISs of HIV-1 in cases of coinfection were more frequently located in intergenic regions and transcriptionally silent regions, compared with HIV-1 mono-infected individuals. CONCLUSION: HIV-1/HTLV-1 coinfection makes an impact on the distribution of viral ISs and the clonality of virus-infected cells and thus may alter the risks of both HTLV-1- and HIV-1-associated disease.
Date Issued
2022-01-15
Date Acceptance
2021-04-01
Citation
Journal of Infectious Diseases, 2022, 225, pp.317-326
ISSN
0022-1899
Publisher
Oxford University Press
Start Page
317
End Page
326
Journal / Book Title
Journal of Infectious Diseases
Volume
225
Copyright Statement
© The Author(s) 2021. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: journals.permissions@oup.com.
This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model). This is a pre-copy-editing, author-produced version of an article accepted for publication in The Journal of Infectious Diseases following peer review. The definitive publisher-authenticated version Hiroo Katsuya, Lucy B M Cook, Aileen G Rowan, Anat Melamed, Jocelyn Turpin, Jumpei Ito, Saiful Islam, Paola Miyazato, Benjy Jek Yang Tan, Misaki Matsuo, Toshikazu Miyakawa, Hirotomo Nakata, Shuzo Matsushita, Graham P Taylor, Charles R M Bangham, Shinya Kimura, Yorifumi Satou, Clonality of HIV-1 and HTLV-1 infected cells in naturally coinfected individuals, The Journal of Infectious Diseases, 2021;, jiab202, https://doi.org/10.1093/infdis/jiab202
This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model). This is a pre-copy-editing, author-produced version of an article accepted for publication in The Journal of Infectious Diseases following peer review. The definitive publisher-authenticated version Hiroo Katsuya, Lucy B M Cook, Aileen G Rowan, Anat Melamed, Jocelyn Turpin, Jumpei Ito, Saiful Islam, Paola Miyazato, Benjy Jek Yang Tan, Misaki Matsuo, Toshikazu Miyakawa, Hirotomo Nakata, Shuzo Matsushita, Graham P Taylor, Charles R M Bangham, Shinya Kimura, Yorifumi Satou, Clonality of HIV-1 and HTLV-1 infected cells in naturally coinfected individuals, The Journal of Infectious Diseases, 2021;, jiab202, https://doi.org/10.1093/infdis/jiab202
Sponsor
Wellcome Trust
Identifier
https://www.ncbi.nlm.nih.gov/pubmed/33844021
PII: 6222242
Grant Number
100291/Z/12/Z
Subjects
HIV-1
HTLV-1
High-throughput sequencing
Integration site analysis
coinfection
Publication Status
Published
Coverage Spatial
United States
Date Publish Online
2021-04-12