Purpose: This study aims to assess gender-based differences on farmers’ perception of impacts and vulnerability to climate change and the implementation of adaptation strategies in the Pra River Basin of Ghana, while also providing lessons for other Sub-Saharan nations and regions with similar conditions. || Design/methodology/approach: The study used semi-structured interviews and questionnaires to collect data from 344 farmers, 64 participants in focus group discussions and 6 agriculture extension officers (key informants) from 10 districts in the Pra River Basin of Ghana. || Findings: Results showed several differences in how climate change is perceived and tackled by male and female genders. In the perception of male farmers, for example, they were found to be more vulnerable to increased temperature, and changes in rainfall and growing season, whereas female farmers on average were considered to be less resilient to floods and droughts for different reasons. Moreover, floods posed higher risks to farming than other climate change impacts. Gender roles had a significant correlation with the type of adaptation strategies practised. Men adopted agrochemicals more often than women, as an adaptation strategy. || Research limitations/implications: Gender-differentiated interventions should be incorporated in the national climate change action plan for sustainable development in a rain-fed agricultural economy such as Ghana. The study recommends several actions to promote gender equity in the assessed region. || Originality/value: This research assessed the gender differentials in climate trends, impact, vulnerability and adaptation based on primary data collected between April and May 2019 and compared the results with climate data in the basin for the period 1991–2014. It is an empirical study focused on primary data analysis obtained in loco by authors, involving approximately 400 participants.