Dear Spiral user, We’re pleased to introduce Helix, https://helix.imperial.ac.uk/ Imperial’s new repository for research datasets. Please use Helix for all future dataset deposits. There is no change to Spiral - continue depositing as usual. For queries, contact the Research Data Management team at rdm-enquiries@imperial.ac.uk. Kind regards, the Spiral Team. The Spiral team
Structural Basis of Host Autophagy-related Protein 8 (ATG8) Binding by the Irish Potato Famine Pathogen Effector Protein PexRD54
File(s)
Author(s)
Maqbool, A
Hughes, RK
Dagdas, YF
Tregidgo, N
Zess, E
Type
Journal Article
Abstract
Filamentous plant pathogens deliver effector proteins to host cells to promote infection. The Phytophthora infestans RXLR-type effector PexRD54 binds potato ATG8 via its ATG8 family-interacting motif (AIM) and perturbs host-selective autophagy. However, the structural basis of this interaction remains unknown. Here, we define the crystal structure of PexRD54, which includes a modular architecture, including five tandem repeat domains, with the AIM sequence presented at the disordered C terminus. To determine the interface between PexRD54 and ATG8, we solved the crystal structure of potato ATG8CL in complex with a peptide comprising the effector's AIM sequence, and we established a model of the full-length PexRD54-ATG8CL complex using small angle x-ray scattering. Structure-informed deletion of the PexRD54 tandem domains reveals retention of ATG8CL binding in vitro and in planta This study offers new insights into structure/function relationships of oomycete RXLR effectors and how these proteins engage with host cell targets to promote disease.
Date Issued
2016-07-25
Date Acceptance
2016-07-18
Citation
Journal of Biological Chemistry, 2016, 291 (38), pp.20270-20282
URI
DOI
ISSN
1083-351X
Publisher
American Society for Biochemistry and Molecular Biology
Start Page
20270
End Page
20282
Journal / Book Title
Journal of Biological Chemistry
Volume
291
Issue
38
Copyright Statement
© 2016 The American Society for Biochemistry and Molecular Biology, Inc. Final version free via Creative Commons CC-BY license.
License URL
Sponsor
Biotechnology and Biological Sciences Research Council (BBSRC)
Grant Number
BB/M002462/1
Subjects
autophagy
effector protein
host-pathogen interaction
plant molecular biology
plant pathogen
protein structure
protein-protein interaction
Biochemistry & Molecular Biology
06 Biological Sciences
11 Medical And Health Sciences
03 Chemical Sciences
Publication Status
Published