Bloch surface wave label-free and fluorescence platform for the detection of VEGF biomarker in biological matrices
File(s)
Accepted version
Author(s)
Type
Journal Article
Abstract
We report on the detection of an angiogenic molecule Vascular Endothelial Growth Factor (VEGF) in different biological matrices by means of a new integrated biosensing platform exploiting the properties of Bloch surface waves. The new platform takes advantage of a tandem configuration, in which both label-free and enhanced fluorescence detection are implemented. Specifically designed one dimensional photonic crystals were deposited directly on disposable and low cost plastic biochips. A direct sandwich immunoassay was used to detect VEGF in buffer, cell culture supernatant and human plasma at low concentration (ng/mL). The platform enabled the detection of VEGF in all three matrices with high resolution, fast turnaround time (30 min) and in close agreement with the results of enzyme linked immunosorbent assays.
Date Issued
2017-09-06
Date Acceptance
2017-09-04
Citation
Sensors and Actuators B: Chemical, 2017, 255, pp.2143-2150
ISSN
0925-4005
Publisher
Elsevier
Start Page
2143
End Page
2150
Journal / Book Title
Sensors and Actuators B: Chemical
Volume
255
Copyright Statement
© 2017, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/
Subjects
Science & Technology
Physical Sciences
Technology
Chemistry, Analytical
Electrochemistry
Instruments & Instrumentation
Chemistry
Biosensor platform
Bloch surface waves
Label-free optical sensor
Enhanced fluorescence
Tumor biomarker
Complex matrices
DIMENSIONAL PHOTONIC CRYSTALS
ENDOTHELIAL GROWTH-FACTOR
OPTICAL BIOSENSORS
PLASMON RESONANCE
BREAST-CANCER
SENSOR
PERFORMANCE
Publication Status
Published