Deep orange gene editing triggers temperature-sensitive lethal phenotypes in Ceratitis capitata
Author(s)
Type
Journal Article
Abstract
The Mediterranean fruit fly, Ceratitis capitata, is a significant agricultural pest managed through area-wide integrated pest management (AW-IPM) including a sterile insect technique (SIT) component. Male-only releases increase the efficiency and cost-effectiveness of SIT programs, which can be achieved through the development of genetic sexing strains (GSS). The most successful GSS developed to date is the C. capitata VIENNA 8 GSS, constructed using classical genetic approaches and an irradiation-induced translocation with two selectable markers: the white pupae (wp) and temperature-sensitive lethal (tsl) genes. However, currently used methods for selecting suitable markers and inducing translocations are stochastic and non-specific, resulting in a laborious and time-consuming process. Recent efforts have focused on identifying the gene(s) and the causal mutation(s) for suitable phenotypes, such as wp and tsl, which could be used as selectable markers for developing a generic approach for constructing GSS. The wp gene was recently identified, and efforts have been initiated to identify the tsl gene. This study investigates Ceratitis capitata deep orange (Ccdor) as a tsl candidate gene and its potential to induce tsl phenotypes.
Date Issued
2024-02-01
Date Acceptance
2024-01-17
Citation
BMC Biotechnology, 2024, 24
ISSN
1472-6750
Publisher
BMC
Journal / Book Title
BMC Biotechnology
Volume
24
Copyright Statement
© The Author(s) 2024. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
License URL
Identifier
https://www.ncbi.nlm.nih.gov/pubmed/38302991
PII: 10.1186/s12896-024-00832-x
Subjects
Biotechnology & Applied Microbiology
DIPTERA
DROSOPHILA-MELANOGASTER
IDENTIFICATION
Life Sciences & Biomedicine
Mediterranean fruit fly
MEDITERRANEAN FRUIT-FLY
MUTATIONS
NOTCH
PACKING
PROTEIN
Science & Technology
SEXING STRAINS
Sterile insect technique
STERILE INSECT TECHNIQUE
Temperature sensitivity
Tephritidae
White pupae
Publication Status
Published
Coverage Spatial
England
Article Number
7
Date Publish Online
2024-02-01