Yeast transformation efficiency is enhanced by TORC1- and eisosome-dependent signalling
File(s)
Published version
Author(s)
Yu, Sheng-Chun
Kuemmel, Florian
Skoufou-Papoutsaki, Maria-Nefeli
Spanu, PD
Type
Journal Article
Abstract
Transformation of baker's yeast (Saccharomyces cerevisiae) plays a key role in several experimental techniques, yet the molecular mechanisms underpinning transformation are still unclear. The addition of amino acids to the growth and transformation medium increases transformation efficiency. Here, we show that target of rapamycin complex 1 (TORC1) activated by amino acids enhances transformation via ubiquitin‐mediated endocytosis. We created mutants of the TORC1 pathway, alpha‐arrestins, and eisosome‐related genes. Our results demonstrate that the TORC1‐Npr1‐Art1/Rsp5 pathway regulates yeast transformation. Based on our previous study, activation of this pathway results in up to a 200‐fold increase in transformation efficiency, or greater. Additionally, we suggest DNA may be taken up by domains at the membrane compartment of Can1 (MCC) in the plasma membrane formed by eisosomes. Yeast studies on transformation could be used as a platform to understand the mechanism of DNA uptake in mammalian systems, which is clinically relevant to optimize gene therapy.
Date Issued
2019-05-01
Date Acceptance
2018-08-10
Citation
Microbiology Open, 2019, 8 (5)
ISSN
2045-8827
Publisher
Wiley
Journal / Book Title
Microbiology Open
Volume
8
Issue
5
Copyright Statement
© 2018 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.
This is an open access article under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
This is an open access article under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
License URL
Sponsor
Biotechnology and Biological Sciences Research Council (BBSRC)
Grant Number
BB/M000710/1
Subjects
Science & Technology
Life Sciences & Biomedicine
Microbiology
central metabolism
endocytosis
extracellular DNA
gene transfer
GAP1 PERMEASE UBIQUITYLATION
UBIQUITIN-LIGASE
INTRACELLULAR TRAFFICKING
SACCHAROMYCES-CEREVISIAE
PHEROMONE RESPONSE
PKH-YPK
PROTEIN
ENDOCYTOSIS
STRESS
PATHWAY
central metabolism
endocytosis
extracellular DNA
gene transfer
Publication Status
Published
Article Number
e730
Date Publish Online
2018-10-11