Amphiphilic DNA nanostructures for bottom-up synthetic biology
File(s)d1cc04311k.pdf (6.95 MB)
Published version
Author(s)
Rubio-Sanchez, Roger
Fabrini, Giacomo
Cicuta, Pietro
Di Michele, Lorenzo
Type
Journal Article
Abstract
DNA nanotechnology enables the construction of sophisticated biomimetic nanomachines that are increasingly central to the growing efforts of creating complex cell-like entities from the bottom-up. DNA nanostructures have been proposed as both structural and functional elements of these artificial cells, and in many instances are decorated with hydrophobic moieties to enable interfacing with synthetic lipid bilayers or regulating bulk self-organisation. In this feature article we review recent efforts to design biomimetic membrane-anchored DNA nanostructures capable of imparting complex functionalities to cell-like objects, such as regulated adhesion, tissue formation, communication and transport. We then discuss the ability of hydrophobic modifications to enable the self-assembly of DNA-based nanostructured frameworks with prescribed morphology and functionality, and explore the relevance of these novel materials for artificial cell science and beyond. Finally, we comment on the yet mostly unexpressed potential of amphiphilic DNA-nanotechnology as a complete toolbox for bottom-up synthetic biology – a figurative and literal scaffold upon which the next generation of synthetic cells could be built.
Date Issued
2021-10-28
Date Acceptance
2021-10-28
Citation
Chemical Communications, 2021, 57 (95), pp.12725-12740
ISSN
1359-7345
Publisher
Royal Society of Chemistry
Start Page
12725
End Page
12740
Journal / Book Title
Chemical Communications
Volume
57
Issue
95
Copyright Statement
© The Royal Society of Chemistry 2021. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.
Identifier
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000715929300001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
Subjects
Science & Technology
Physical Sciences
Chemistry, Multidisciplinary
Chemistry
NEUTROPHIL EXTRACELLULAR TRAPS
NUCLEIC-ACID JUNCTIONS
LIPID-MEMBRANES
SELF-ORGANIZATION
ARTIFICIAL CELLS
GIANT VESICLES
ORIGAMI
ADHESION
KINETICS
DYNAMICS
Publication Status
Published
OA Location
https://pubs.rsc.org/en/content/articlepdf/2021/cc/d1cc04311k
Date Publish Online
2021-10-28