Detection of colistin resistance in Salmonella enterica using MALDIxin test on the routine MALDI Biotyper Sirius mass spectrometer
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Published version
Author(s)
Type
Journal Article
Abstract
Resistance to polymyxins in most Gram-negative bacteria arises from chemical modifications to the lipid A portion of their lipopolysaccharide (LPS) mediated by chromosomally-encoded mutations or the recently discovered plasmid-encoded mcr genes that have further complicated the landscape of colistin resistance. Currently, minimal inhibitory concentration (MIC) determination by broth microdilution, the gold standard for the detection of polymyxin resistance, is time consuming (24 hours) and challenging to perform in clinical and veterinatryveterinary laboratories. Here we present the use of the MALDIxin to detect colistin resistant Salmonella enterica using the MALDxin test on the routine matrix-assisted laser desorption ionization (MALDI) Biotyper Sirius system.
Date Issued
2020-06-03
Date Acceptance
2020-05-05
Citation
Frontiers in Microbiology, 2020, 11, pp.1-6
ISSN
1664-302X
Publisher
Frontiers Media
Start Page
1
End Page
6
Journal / Book Title
Frontiers in Microbiology
Volume
11
Copyright Statement
© 2020 Dortet, Bonnin, Le Hello, Fabre, Bonnet, Kostrzewa, Filloux and Larrouy-Maumus. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY)(http://creativecommons.org/licenses/by/4.0/). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Sponsor
Wellcome Trust
Identifier
https://www.frontiersin.org/articles/10.3389/fmicb.2020.01141/full
Grant Number
105603/Z/14/Z
Subjects
MALDI mass spectrometry
Salmonella enterica
colistin
diagno stic
lipid A
0502 Environmental Science and Management
0503 Soil Sciences
0605 Microbiology
Publication Status
Published
Article Number
1141
Date Publish Online
2020-06-03