Lipid function and importance in disease are being rediscovered due to modern advancements in chemical
analysis. RP–UPLC–TOF–MSE is now the lipidomics tool of choice and can provide the demanded specificity
for detecting the great diversity of the lipidome. It can offer simplicity, rapidity, robustness and high
throughputness, without the need for further optimization in current sample preparation protocols. This
method can cover the major lipid categories with the ability to detect several corresponding subclasses.
It can deliver adequate information for deciphering fatty chain length, unsaturation and regioisomerism.
It has enabled the detection of a vast number of lipids, of which more than 250 are reported here. These
lipids were detected from applications in a variety of biological matrices and species.