Multilayer genetic circuits for dynamic regulation of metabolic pathways
File(s)
Author(s)
Type
Journal Article
Abstract
The dynamic regulation of metabolic pathways is based on changes in external signals and endogenous changes in gene expression levels and has extensive applications in the field of synthetic biology and metabolic engineering. However, achieving dynamic control is not trivial, and dynamic control is difficult to obtain using simple, single-level, control strategies because they are often affected by native regulatory networks. Therefore, synthetic biologists usually apply the concept of logic gates to build more complex and multilayer genetic circuits that can process various signals and direct the metabolic flux toward the synthesis of the molecules of interest. In this review, we first summarize the applications of dynamic regulatory systems and genetic circuits and then discuss how to design multilayer genetic circuits to achieve the optimal control of metabolic fluxes in living cells.
Date Issued
2021-07-16
Date Acceptance
2021-07-01
Citation
ACS Synthetic Biology, 2021, 10 (7), pp.1587-1597
ISSN
2161-5063
Publisher
American Chemical Society
Start Page
1587
End Page
1597
Journal / Book Title
ACS Synthetic Biology
Volume
10
Issue
7
Copyright Statement
© 2021 American Chemical Society. This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Synthetic Biology, after peer review and technical editing by the publisher. To access the final edited and published work see https://v2.sherpa.ac.uk/id/publication/21296
Identifier
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000674933400002&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
Subjects
Science & Technology
Life Sciences & Biomedicine
Biochemical Research Methods
Biochemistry & Molecular Biology
dynamic regulation
logic gate
multilayer gene circuits
pathway engineering
synthetic biology
LOGIC GATES
DESIGN
BIOSENSORS
STATE
FLUX
ACTIVATION
PRINCIPLES
PROMOTER
TOOLS
Publication Status
Published
Date Publish Online
2021-07-02