Loop-mediated isothermal amplification-integrated CRISPR methods for infectious disease diagnosis at point of care
Author(s)
Yigci, Defne
Atceken, Nazente
Yetisen, Ali K
Tasoglu, Savas
Type
Journal Article
Abstract
Infectious diseases continue to pose an imminent threat to global public health, leading to high numbers of deaths every year and disproportionately impacting developing countries where access to healthcare is limited. Biological, environmental, and social phenomena, including climate change, globalization, increased population density, and social inequity, contribute to the emergence of novel communicable diseases. Rapid and accurate diagnoses of infectious diseases are essential to preventing the transmission of infectious diseases. Although some commonly used diagnostic technologies provide highly sensitive and specific measurements, limitations including the requirement for complex equipment/infrastructure and refrigeration, the need for trained personnel, long sample processing times, and high cost remain unresolved. To ensure global access to affordable diagnostic methods, loop-mediated isothermal amplification (LAMP) integrated clustered regularly interspaced short palindromic repeat (CRISPR) based pathogen detection has emerged as a promising technology. Here, LAMP-integrated CRISPR-based nucleic acid detection methods are discussed in point-of-care (PoC) pathogen detection platforms, and current limitations and future directions are also identified.
Date Issued
2023-11-21
Date Acceptance
2023-09-26
Citation
ACS Omega, 2023, 8 (46), pp.43357-43373
ISSN
2470-1343
Publisher
American Chemical Society
Start Page
43357
End Page
43373
Journal / Book Title
ACS Omega
Volume
8
Issue
46
Copyright Statement
© 2023 The Authors. Published by American Chemical Society. This publication is licensed under CC-BY 4.0 .
License URL
Identifier
https://www.ncbi.nlm.nih.gov/pubmed/38027359
Subjects
Chemistry
Chemistry, Multidisciplinary
COVID-19
DETECTION PLATFORM
LAMP
MICROFLUIDICS
NUCLEIC-ACID DETECTION
Physical Sciences
RAPID DETECTION
SARS-COV-2 DETECTION
Science & Technology
TECHNOLOGIES
TESTS
VISUAL DETECTION
Publication Status
Published
Coverage Spatial
United States
Date Publish Online
2023-11-07