Calcium phosphate substrates with emulsion-derived roughness: processing, characterisation and interaction with human mesenchymal stem cells
File(s)
Accepted version
Author(s)
Type
Journal Article
Abstract
Calcium phosphates (CaP) have been the subject of several studies that often lack a systematic approach to understanding how their properties affect biological response. CaP particles functionalised with a pH-responsive polymer (BCS) were used to prepare microporous substrates (porosity between 70 and 75% and pore sizes of 5–20 μm) through the aggregation of oil-in-water emulsions by controlling solid loading, emulsification energy, pH, drying and sintering conditions. The combined effect of surface roughness (roughness amplitude, Ra between 0.9–1.7 μm) and chemistry (varying Hydroxyapatite/β-Tricalcium phosphate ratio) on human mesenchymal stem cells was evaluated. HA substrates stimulated higher cell adhesion and proliferation (especially with lower Ra), but cell area increased with β-TCP content. The effect of surface roughness depended of chemistry: HA promoted higher mineralising activity when Ra ∼ 1.5 μm, whereas β-TCP substrates stimulated a more osteogenic profile when Ra ∼ 1.7 μm. A novel templating method to fabricate microporous CaP substrates was developed, opening possibilities for bone substitutes with controlled features.
Date Issued
2017-06-27
Date Acceptance
2017-06-24
Citation
Journal of the European Ceramic Society, 2017, 38 (3), pp.949-961
ISSN
0955-2219
Publisher
Elsevier
Start Page
949
End Page
961
Journal / Book Title
Journal of the European Ceramic Society
Volume
38
Issue
3
Copyright Statement
© 2017 Elsevier Ltd. All rights reserved. This manuscript is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/
Sponsor
Commission of the European Communities
Grant Number
289958
Subjects
Science & Technology
Technology
Materials Science, Ceramics
Materials Science
Calcium phosphates
Emulsions
Microporosity
Surface roughness
Human mesenchymal stem cells
BETA-TRICALCIUM PHOSPHATE
PICKERING EMULSIONS
SURFACE-TOPOGRAPHY
HYDROXYAPATITE NANOPARTICLES
SCAFFOLDS
DIFFERENTIATION
OSTEOBLAST
PARTICLES
CERAMICS
ADHESION
0912 Materials Engineering
Materials
Publication Status
Published