Condensin I subunit Cap-G is essential for proper gene expression during the maturation of post-mitotic neurons
File(s)
Published version
Supplementary information
Author(s)
Type
Journal Article
Abstract
Condensin complexes are essential for mitotic chromosome assembly and segregation during cell divisions, however, little is known about their functions in post-mitotic cells. Here we report a role for the condensin I subunit Cap-G in Drosophila neurons. We show that, despite not requiring condensin for mitotic chromosome compaction, post-mitotic neurons express Cap-G. Knockdown of Cap-G specifically in neurons (from their birth onwards) results in developmental arrest, behavioural defects, and dramatic gene expression changes, including reduced expression of a subset of neuronal genes and aberrant expression of genes that are not normally expressed in the developing brain. Knockdown of Cap-G in mature neurons results in similar phenotypes but to a lesser degree. Furthermore, we see dynamic binding of Cap-G at distinct loci in progenitor cells and differentiated neurons. Therefore, Cap-G is essential for proper gene expression in neurons and plays an important role during the early stages of neuronal development.
Date Issued
2020-04-07
Date Acceptance
2020-04-06
Citation
eLife, 2020, 9
ISSN
2050-084X
Publisher
eLife Sciences Publications Ltd
Journal / Book Title
eLife
Volume
9
Copyright Statement
© 2020, Hassan et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
Sponsor
Wellcome Trust
Biotechnology and Biological Sciences Research Council (BBSRC)
Grant Number
104567/Z/14/Z
BB/P017924/1
Subjects
D. melanogaster
DamID
condensin
development
developmental biology
neuron
post-mitotic
0601 Biochemistry and Cell Biology
Publication Status
Published
Article Number
ARTN e55159