Generating tooth organoids using defined bioorthogonally cross-linked hydrogels
Author(s)
Type
Journal Article
Abstract
Generating teeth in vitro requires mimicking tooth developmental processes. Biomaterials are essential to support 3D tooth organoid formation, but their properties must be finely tuned to achieve the required biomimicry for tooth development. For the first time, we used bioorthogonally cross-linked hydrogels as defined 3D matrixes for tooth developmental engineering, and we highlighted how their properties play a pivotal role in enabling 3D tooth organoid formation in vitro. We prepared hydrogels by mixing gelatin precursors modified either with tetrazine (Tz) or norbornene (Nb) moieties. We tuned the hydrogel properties (E = 2–7 kPa; G′ = 500–1500 Pa) by varying the gelatin concentration (8% vs 12% w/V) and stoichiometric ratio (Tz:Nb = 1 vs 0.5). We encapsulated dental epithelial-mesenchymal cell pellets in a library of hydrogels and identified a hydrogel formulation that enabled successful growth kinetics and morphogenesis of tooth germs, introducing a defined tunable platform for tooth organoid engineering and modeling.
Date Issued
2024-12-17
Date Acceptance
2024-11-08
ISSN
2161-1653
Publisher
American Chemical Society
Start Page
1620
End Page
1626
Journal / Book Title
ACS Macro Letters
Volume
13
Issue
12
Copyright Statement
© 2024 The Authors. Published by American Chemical Society. This publication is licensed under
CC-BY 4.0 .
CC-BY 4.0 .
Identifier
https://pubs.acs.org/doi/10.1021/acsmacrolett.4c00520
Publication Status
Published
Date Publish Online
2024-11-12