Understanding current distribution in operating Vanadium redox flow batteries (VRFBs) is vital for improving battery design and performance as the non-uniform current density distribution is known as a major cause of the corrosion and side reactions in VRFBs. However, the existing method for current density measurement in the VRFB with flow field is complex and the rib conductive structure of graphite plate is changed which reduces the measurement accuracy and reliability. In this study, we propose a novel pluggable current collector for in-operando current distribution measurements which greatly simplifies the current density distribution measurement process in VRFBs with flow field. The new method relies on eight pluggable graphite units in a flow plate frame to replicate the actual serpentine flow field structure, thereby providing higher measurement reliability compared to the existing methods which are mostly invasive. Using the new method, current density distributions at different electrode compression ratios (CR) and inflow electrolyte rates are investigated. It is found that insufficient supplemental reactant in electrode border region is the main reason for the uneven current density distribution and the current density non-uniformity is intensified in the initial and final stages of cycling process. Increasing CR can improve the current density in the electrode core region. The overall current density uniformity in a VRFB with serpentine flow field can be enhanced by increasing the inflow electrolyte rate. The developed method is believed to facilitate the understanding of the coupled local reactions and transport processes and guide on the future design of VRFBs.