Benchtop proton NMR spectroscopy for high-throughput lipoprotein quantification in human serum and plasma
Author(s)
Type
Journal Article
Abstract
We report the successful development and translation of high-field nuclear magnetic resonance (NMR) based comprehensive lipoprotein analysis to routine benchtop systems. This demonstrates the potential to reimagine population level cardiovascular disease risk analysis and individual level screening based on blood sampling. Using a quantitative calibration approach, we obtained stable and reproducible results from multiple sites, despite reduced spectral dispersion and sensitivity at lower field strengths. Our study shows that 25 out of 28 major lipoprotein parameters, including key cardiometabolic risk markers, were faithfully measured using benchtop NMR systems within 15 min. This development has significant implications for making a powerful diagnostic tool widely available, enhancing the potential for longitudinal personalized medicine through molecular phenotyping in the clinic.
Date Issued
2025-04-01
Date Acceptance
2025-03-05
Citation
Analytical Chemistry, 2025, 97 (12), pp.6399-6409
ISSN
0003-2700
Publisher
American Chemical Society (ACS)
Start Page
6399
End Page
6409
Journal / Book Title
Analytical Chemistry
Volume
97
Issue
12
Copyright Statement
© 2025 The Authors. Published by American Chemical Society. This publication is licensed under CC-BY-NC-ND 4.0 .
Identifier
https://www.ncbi.nlm.nih.gov/pubmed/40095953
Subjects
Humans
Lipoproteins
Proton Magnetic Resonance Spectroscopy
Magnetic Resonance Spectroscopy
High-Throughput Screening Assays
Publication Status
Published
Coverage Spatial
United States
Date Publish Online
2025-03-17