Snapshot imprinting: Rapid identification of cancer cell surface proteins and epitopes using molecularly imprinted polymers
File(s)
Published version
Author(s)
Type
Journal Article
Abstract
Proteomic mapping of cell surfaces is an invaluable tool for drug development and clinical diagnostics. This work describes a new ‘snapshot imprinting’ method designed to obtain proteomic maps of cell surfaces, with the aim of identifying cell surface markers and epitopes for diagnostic and therapeutic applications. The analysis of two cancer cell lines, HN5 and MDA-MB-468, is described herein as a proof of concept, along with the selective targeting of three identified epitopes of epidermal growth factor receptor using molecularly imprinted polymer nanoparticles. 438 proteins were identified using this technique, with 283 considered to be transmembrane or extracellular proteins. The major advantage of the molecular imprinting approach developed here is the ability to analyse cell surface proteins without tedious fractionation, affinity separation or labelling. We believe that this system of protein analysis may provide a basic molecular diagnostics toolbox for precise, personalised treatment of cancer and other diseases.
Date Issued
2021-12-01
Date Acceptance
2021-09-18
Citation
Nano Today: an international rapid reviews journal, 2021, 41, pp.1-8
ISSN
1748-0132
Publisher
Elsevier
Start Page
1
End Page
8
Journal / Book Title
Nano Today: an international rapid reviews journal
Volume
41
Copyright Statement
© 2021 The Authors. Published by Elsevier Ltd. This is an open acess article under a CC-BY Attribution License (https://creativecommons.org/licenses/by/4.0/)
License URL
Sponsor
Imperial College Healthcare NHS Trust- BRC Funding
Imperial College Healthcare NHS Trust- BRC Funding
Medical Research Council
Identifier
https://www.sciencedirect.com/science/article/pii/S1748013221002292?via%3Dihub
Grant Number
RDC04 79560
RDC04
Subjects
Science & Technology
Physical Sciences
Technology
Chemistry, Multidisciplinary
Nanoscience & Nanotechnology
Materials Science, Multidisciplinary
Chemistry
Science & Technology - Other Topics
Materials Science
Cancer
Proteomics
Molecularly imprinted polymers
Epitopes
Cells
STEM-CELLS
NANOPARTICLES
PROTEOMICS
SECRETION
HEAD
EGFR
Nanoscience & Nanotechnology
0903 Biomedical Engineering
1007 Nanotechnology
Publication Status
Published
Article Number
ARTN 101304
Date Publish Online
2021-10-05