Nanoceria provides antioxidant and osteogenic properties to mesoporous silica nanoparticles for osteoporosis treatment
File(s)Supplementary Information.docx (1.58 MB) Pinna et al silica nanoceria submission_text.docx (5.3 MB)
Accepted version
Accepted version
Author(s)
Pinna, Alessandra
Baghbaderani, Mohammad Torki
Hernandez, Victoria Vigil
Naruphontjirakul, Parichart
Li, Siwei
Type
Journal Article
Abstract
Osteoporosis, a chronic metabolic bone disease, is the most common cause of fractures. Drugs for treating osteoporosis generally inhibit osteoclast (OC) activity, but are rarely aimed at encouraging new bone growth and often cause severe systemic side effects. Reactive oxygen species (ROS) are one of the key triggers of osteoporosis, by inducing osteoblast (OB) and osteocyte apoptosis and promoting osteoclastogenesis. Here we tested the capability of the ROS-scavenger nanoceria encapsulated within mesoporous silica nanoparticles (Ce@MSNs) to treat osteoporosis using a pre-osteoblast MC3T3-E1 cell monoculture in stressed and normal conditions. Ce@MSNs (diameter of 80 ± 10 nm) were synthesised following a scalable two-step process involving sol-gel and wet impregnation methods. The Ce@MSNs at concentration of 100 μg mL−1 induced a significant reduction in oxidative stress produced by t-butyl hydroperoxide and did not alter cell viability significantly. Confocal microscopy showed that MSNs and Ce@MsNs were internalised into the cytoplasm of the pre-osteoblasts after 24 h but were not in the nucleus, avoiding any DNA and RNA modifications. Ce@MSNs provoked mineralisation of the pre-osteoablasts without osteogenic supplements, which did not occur when the cells were exposed to MSN without nanoceria. In a co-culture system of MC3T3-E1 and RAW264.7 macrophages, the Ce@MSNs exhibited antioxidant capability and stimulated cell proliferation and osteogenic responses without adding osteogenic supplements to the culture. The work brings forward an effective platform based for facile synthesis of Ce@MSNs to interact with both OBs and OCs for treatment of osteoporosis.
Date Issued
2021-02-16
Date Acceptance
2020-12-13
Citation
Acta Biomaterialia, 2021, 122, pp.365-376
ISSN
1742-7061
Publisher
Elsevier
Start Page
365
End Page
376
Journal / Book Title
Acta Biomaterialia
Volume
122
Copyright Statement
© 2021 Elsevier Ltd. All rights reserved. This manuscript is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International Licence http://creativecommons.org/licenses/by-nc-nd/4.0/
Identifier
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000620488200007&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
Subjects
Science & Technology
Technology
Engineering, Biomedical
Materials Science, Biomaterials
Engineering
Materials Science
Nanoparticles
Radical scavengers
Osteoporosis
Bioactive glass
Nanoceria
CERIUM OXIDE NANOPARTICLES
BONE-MINERAL DENSITY
PRIMARY OSTEOBLASTS
OXIDATIVE STRESS
DIFFERENTIATION
BISPHOSPHONATES
CELLS
MECHANISM
GROWTH
Publication Status
Published
Date Publish Online
2021-01-05