Size-Dependent Expression of the Mitotic Activator Cdc25 Suggests a Mechanism of Size Control in Fission Yeast
File(s)
Accepted version
Author(s)
Type
Journal Article
Abstract
Proper cell size is essential for cellular function. Nonetheless, despite more than 100 years of work on the subject, the mechanisms that maintain cell-size homeostasis are largely mysterious [ 1 ]. Cells in growing populations maintain cell size within a narrow range by coordinating growth and division. Bacterial and eukaryotic cells both demonstrate homeostatic size control, which maintains population-level variation in cell size within a certain range and returns the population average to that range if it is perturbed [ 1, 2 ]. Recent work has proposed two different strategies for size control: budding yeast has been proposed to use an inhibitor-dilution strategy to regulate size at the G1/S transition [ 3 ], whereas bacteria appear to use an adder strategy, in which a fixed amount of growth each generation causes cell size to converge on a stable average [ 4–6 ]. Here we present evidence that cell size in the fission yeast Schizosaccharomyces pombe is regulated by a third strategy: the size-dependent expression of the mitotic activator Cdc25. cdc25 transcript levels are regulated such that smaller cells express less Cdc25 and larger cells express more Cdc25, creating an increasing concentration of Cdc25 as cells grow and providing a mechanism for cells to trigger cell division when they reach a threshold concentration of Cdc25. Because regulation of mitotic entry by Cdc25 is well conserved, this mechanism may provide a widespread solution to the problem of size control in eukaryotes.
Date Issued
2017-05-22
Date Acceptance
2017-04-11
Citation
Current Biology, 2017, 27 (10), pp.1491-1497.e4
ISSN
1879-0445
Publisher
Elsevier
Start Page
1491
End Page
1497.e4
Journal / Book Title
Current Biology
Volume
27
Issue
10
Copyright Statement
© 2017, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/
Subjects
Science & Technology
Life Sciences & Biomedicine
Biochemistry & Molecular Biology
Cell Biology
CELL-SIZE
SCHIZOSACCHAROMYCES-POMBE
MESSENGER-RNA
BUDDING-YEAST
QUANTITATIVE-ANALYSIS
NUCLEAR DIVISION
DNA-DAMAGE
HEAT SHOCK
CYCLE
PROTEIN
Publication Status
Published