Fluorescent probe combined with photoelectric analysis technology for detection of Escherichia coli
File(s)
Published version
Author(s)
Type
Journal Article
Abstract
Food safety is facing great challenges in preventing foodborne diseases caused by pathogenic pollution, especially in resource-limited areas. The rapid detection technique of microorganisms, such as immunological methods and molecular biological methods, plays a crucial key in timely bioanalysis and disease treatment strategies. However, it is difficult for these methods to simultaneously meet the criteria of simple operation, high specificity, and sensitivity, as well as low cost. Coconut water is known as the “water of life” in Hainan. It is a refreshing and nutritious beverage which is widely consumed due to its beneficial properties to health. Coconut water processing is an important pillar industry in Hainan. The detection of pathogenic microorganisms, such as Escherichia coli, in coconut water has become an important factor which has restricted the upgrading and development of this industry. Based on the needs of industrial development, we developed a microbial photoelectric detection system which was composed of a fluorescent probe detection reagent and a photoelectric sensor detection device. This system combined microbial enzyme targets, selective fluorescent substrate metabolism characteristics, and a photoelectric sensor signal transduction mechanism, which produce a strong signal with a high signal-to-noise ratio. The microbial detection system developed here has a simple structure, simple and convenient operation, short detecting time (≥2 h), and high sensitivity (1 CFU/mL). This system may also enable early warning and monitoring programs for other pathogenic microorganisms in order to promote the overall competitiveness of the Hainan coconut water industry.
Date Issued
2023-02-01
Date Acceptance
2023-01-12
Citation
Biosensors, 2023, 13 (2)
ISSN
2079-6374
Publisher
MDPI AG
Journal / Book Title
Biosensors
Volume
13
Issue
2
Copyright Statement
© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).
License URL
Identifier
https://www.ncbi.nlm.nih.gov/pubmed/36831916
PII: bios13020150
Subjects
BIOSENSORS
Chemistry
Chemistry, Analytical
Escherichia coli
fluorescent probe
FOOD
Hainan coconut water
Instruments & Instrumentation
microbial photoelectric detection system
MUG
Nanoscience & Nanotechnology
PATHOGENS
photoelectric analysis
Physical Sciences
Science & Technology
Science & Technology - Other Topics
Technology
Publication Status
Published
Coverage Spatial
Switzerland
Article Number
150
Date Publish Online
2023-01-17