Towards development of fibre-optic surface enhanced Raman spectroscopy probes using 2-photon polymerisation for rapid detection of bacteria
File(s)
Author(s)
Kim, Jang Ah
Wales, Dominic J
Thompson, Alexander J
Yang, Guang-Zhong
Type
Conference Paper
Abstract
In this study, a variety of direct laser written surface-enhanced Raman spectroscopy (SERS) micro-structures, designed for bacteria detection, are presented. Various SERS micro-structures were designed to achieve both a high density of plasmonic hot spots and a strong probability of interaction between the hot spots and the target bacterial cells. Twophoton polymerization was used for initial fabrication of the polymeric skeletons of the SERS micro-structures, which were then coated with a 50 nm-thick gold layer via e-beam evaporation. The micro-structures were fabricated on glass coverslips and were assessed using a confocal Raman microscope. To this end, Rhodamine 6G was used as an analyte under 785 nm laser illumination. The optimal SERS micro-structures showed approximately 7×103 enhancement in Raman signal (analytical enhancement factor, AEF) at a wavenumber of 600 cm-1. Real-time detection of E. coli in solution was demonstrated using the fabricated SERS platform with low laser powers and a short acquisition time (785 nm, 5 mW, 50 ms).
Date Issued
2019-03-07
Date Acceptance
2019-02-02
Citation
Proceedings of SPIE: Plasmonics in Biology and Medicine XVI, 2019
ISBN
9781510624306
ISSN
0277-786X
Publisher
SPIE
Journal / Book Title
Proceedings of SPIE: Plasmonics in Biology and Medicine XVI
Copyright Statement
© 2019 SPIE.
Sponsor
Engineering & Physical Science Research Council (EPSRC)
Grant Number
EP/P012779/1
Source
Plasmonics in Biology and Medicine XVI
Publication Status
Published
Start Date
2019-02-02
Finish Date
2019-02-07
Coverage Spatial
San Francisco, California, United States
Date Publish Online
2019-03-07