The blast pathogen effector AVR-Pik binds and stabilizes rice heavy metal-associated (HMA) proteins to co-opt their function in immunity
File(s)
Published version
Author(s)
Type
Journal Article
Abstract
Intracellular nucleotide-binding domain and leucine-rich repeat-containing (NLR) receptors play crucial roles in immunity across multiple domains of life. In plants, a subset of NLRs contain noncanonical integrated domains that are thought to have evolved from host targets of pathogen effectors to serve as pathogen baits. However, the functions of host proteins with similarity to NLR integrated domains and the extent to which they are targeted by pathogen effectors remain largely unknown. Here, we show that the blast fungus effector AVR-Pik binds a subset of related rice proteins containing a heavy metal-associated (HMA) domain, one of the domains that has repeatedly integrated into plant NLR immune receptors. We find that AVR-Pik binding stabilizes the rice small HMA (sHMA) proteins OsHIPP19 and OsHIPP20. Knockout of OsHIPP20 causes enhanced disease resistance towards the blast pathogen, indicating that OsHIPP20 is a susceptibility gene (S-gene). We propose that AVR-Pik has evolved to bind HMA domain proteins and co-opt their function to suppress immunity. Yet this binding carries a trade-off, it triggers immunity in plants carrying NLR receptors with integrated HMA domains.
Editor(s)
Wilson, Richard A
Date Issued
2024-11-18
Date Acceptance
2024-10-07
Citation
PLoS Pathogens, 2024, 20 (11)
ISSN
1553-7366
Publisher
Public Library of Science (PLoS)
Journal / Book Title
PLoS Pathogens
Volume
20
Issue
11
Copyright Statement
© 2024 Oikawa et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
License URL
Identifier
https://www.ncbi.nlm.nih.gov/pubmed/39556648
PII: PPATHOGENS-D-24-00404
Subjects
ALLOWS
BIOLOGY
DISEASE
DOMAINS
GENES
Life Sciences & Biomedicine
Microbiology
PAIR
Parasitology
RESISTANCE
Science & Technology
SYNTHESIS SYSTEM
Virology
Publication Status
Published
Coverage Spatial
United States
Article Number
ARTN e1012647
Date Publish Online
2024-11-18