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Potential value of a rapid syndromic multiplex PCR for the diagnosis of native and prosthetic joint infections: a real-world evidence study
File(s)
jbji-9-87-2024.pdf (1.1 MB)
Published version
Author(s)
Pascual, Stéphanie
Noble, Brooklyn
Ahmad-Saeed, Nusreen
Aldridge, Catherine
Ambretti, Simone
Type
Journal Article
Abstract
Introduction: The BIOFIRE Joint Infection (JI) Panel is a diagnostic tool that uses multiplex-PCR testing to detect microorganisms in synovial fluid specimens from patients suspected of having septic arthritis (SA) on native joints or prosthetic joint infections (PJIs). Methods: A study was conducted across 34 clinical sites in 19 European and Middle Eastern countries from March 2021 to June 2022 to assess the effectiveness of the BIOFIRE JI Panel. Results: A total of 1527 samples were collected from patients suspected of SA or PJI, with an overall agreement of 88.4 % and 85 % respectively between the JI Panel and synovial fluid cultures (SFCs). The JI Panel detected more positive samples and microorganisms than SFC, with a notable difference on Staphylococcus aureus, Streptococcus species, Enterococcus faecalis, Kingella kingae, Neisseria gonorrhoeae, and anaerobic bacteria. The study found that the BIOFIRE JI Panel has a high utility in the real-world clinical setting for suspected SA and PJI, providing diagnostic results in approximately 1 h. The user experience was positive, implying a potential benefit of rapidity of results' turnover in optimising patient management strategies. Conclusion: The study suggests that the BIOFIRE JI Panel could potentially optimise patient management and antimicrobial therapy, thus highlighting its importance in the clinical setting.
Date Issued
2024
Date Acceptance
2023-12-01
Citation
Journal of Bone and Joint Infection, 2024, 9 (1), pp.87-97
URI
URL
DOI
ISSN
2206-3552
Publisher
Copernicus Publications
Start Page
87
End Page
97
Journal / Book Title
Journal of Bone and Joint Infection
Volume
9
Issue
1
Copyright Statement
© Author(s) 2024. This work is distributed under
the Creative Commons Attribution 4.0 License.
License URL
Identifier
https://www.ncbi.nlm.nih.gov/pubmed/38601005
Publication Status
Published
Coverage Spatial
Germany
Date Publish Online
2024-02-28