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Sulfated alginate reduces pericapsular fibrotic overgrowth on encapsulated cGMP-compliant hPSC-hepatocytes in mice
File(s)
Author(s)
Syanda, Adam M
Kringstad, Vera I
Blackford, Samuel JI
Kjesbu, Joachim S
Ng, Soon Seng
Type
Journal Article
Abstract
Intra-peritoneal placement of alginate encapsulated human induced pluripotent stem cell-derived hepatocytes (hPSC-Heps) represents a potential new bridging therapy for acute liver failure. One of the rate-limiting steps that needs to be overcome to make such a procedure more efficacious and safer is to reduce the accumulation of fibrotic tissue around the encapsulated cells to allow the free passage of relevant molecules in and out for metabolism. Novel chemical compositions of alginate afford the possibility of achieving this aim. We accordingly used sulfated alginate and demonstrated that this material reduced fibrotic overgrowth whilst not impeding the process of encapsulation nor cell function. Cumulatively, this suggests sulfated alginate could be a more suitable material to encapsulate hPSC-hepatocyte prior to human use.
Date Issued
2022-03-03
Date Acceptance
2021-12-24
Citation
Frontiers in Bioengineering and Biotechnology, 2022, 9
URI
URL
DOI
ISSN
2296-4185
Publisher
Frontiers Media S.A.
Journal / Book Title
Frontiers in Bioengineering and Biotechnology
Volume
9
Copyright Statement
© 2022 Syanda, Kringstad, Blackford, Kjesbu, Ng, Ma, Xiao, Coron, Rokstad, Modi, Rashid and Strand. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
License URL
Identifier
https://www.ncbi.nlm.nih.gov/pubmed/35308825
PII: 816542
Subjects
acute liver failure
BEADS
BINDING
BIOMATERIALS
Biotechnology & Applied Microbiology
cGMP
CONTROLLED-RELEASE
Engineering
Engineering, Biomedical
hPSC
HYDROGELS
immunogenicity
immunoisolation
ISLETS
Life Sciences & Biomedicine
LIVER-FAILURE
MICROCAPSULES
PFO
pluripotent stem cell-derived hepatocytes
Science & Technology
sulfated alginate
SURVIVAL
Technology
TRANSPLANTATION
Publication Status
Published
Coverage Spatial
Switzerland
Article Number
816542
Date Publish Online
2022-03-03