Microneedle-based nanoporous gold electrochemical sensor for real-time catecholamine detection
Author(s)
Tortolini, Cristina
Cass, Anthony EG
Pofi, Riccardo
Lenzi, Andrea
Antiochia, Riccarda
Type
Journal Article
Abstract
Dopamine (DA), epinephrine (EP), and norepinephrine (NEP) are the main catecholamine of clinical interest, as they play crucial roles in the regulation of nervous and cardiovascular systems and are involved in some brain behaviors, such as stress, panic, anxiety, and depression. Therefore, there is an urgent need for a reliable sensing device able to provide their continuous monitoring in a minimally invasive manner. In this work, the first highly nanoporous gold (h-nPG) microneedle-based sensor is presented for continuous monitoring of catecholamine in interstitial fluid (ISF). The h-nPG microneedle-based gold electrode was prepared by a simple electrochemical self-templating method that involves two steps, gold electrodeposition and hydrogen bubbling at the electrode surface, realized by sweeping the potential between + 0.8 V and 0 V vs Ag/AgCl for 25 scans in a 10 mM HAuCl4 solution containing 2.5 M NH4Cl, and successively applying a fixed potential of − 2 V vs Ag/AgCl for 60 s. The resulting microneedle-based h-nPG sensor displays an interference-free total catecholamine detection expressed as NEP concentration, with a very low LOD of 100 nM, excellent sensitivity and stability, and fast response time (< 4 s). The performance of the h-nPG microneedle array sensor was successively assessed in artificial ISF and in a hydrogel skin model at typical physiological concentrations.
Date Issued
2022-05-01
Date Acceptance
2022-03-04
Citation
Microchimica Acta, 2022, 189 (5)
ISSN
0026-3672
Publisher
Springer
Journal / Book Title
Microchimica Acta
Volume
189
Issue
5
Copyright Statement
© The Author(s) 2022 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
License URL
Identifier
https://www.ncbi.nlm.nih.gov/pubmed/35391571
PII: 10.1007/s00604-022-05260-2
Subjects
ACID
Catecholamine
Chemistry
Chemistry, Analytical
Dopamine
DOPAMINE
Electrochemical sensor
ELECTRODE
Epinephrine
FILM
GRAPHENE OXIDE COMPOSITE
MICROELECTRODES
Microneedles
Nanoporous gold needle
NEUROTRANSMITTERS
Norepinephrine
NOREPINEPHRINE
PHEOCHROMOCYTOMA
Physical Sciences
Science & Technology
SELECTIVE DETERMINATION
Publication Status
Published
Coverage Spatial
Austria
Article Number
180
Date Publish Online
2022-04-07