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Defined conditions for propagation and manipulation of mouse embryonic stem cells
File(s)
dev173146.full-3.pdf (4.71 MB)
Published version
OA Location
Author(s)
Mulas, Carla
Kalkan, Tuzer
von Meyenn, Ferdinand
Leitch, Harry G
Nichols, Jennifer
Type
Journal Article
Abstract
The power of mouse embryonic stem (ES) cells to colonise the developing embryo has revolutionised mammalian developmental genetics and stem cell research. This power is vulnerable, however, to the cell culture environment, deficiencies in which can lead to cellular heterogeneity, adaptive phenotypes, epigenetic aberrations and genetic abnormalities. Here, we provide detailed methodologies for derivation, propagation, genetic modification and primary differentiation of ES cells in 2i or 2i+LIF media without serum or undefined serum substitutes. Implemented diligently, these procedures minimise variability and deviation, thereby improving the efficiency, reproducibility and biological validity of ES cell experimentation.
Date Issued
2019-03-01
Date Acceptance
2019-02-19
Citation
Delepment, 2019, 146 (6)
URI
DOI
ISSN
0950-1991
Publisher
Company of Biologists
Journal / Book Title
Delepment
Volume
146
Issue
6
Copyright Statement
© 2019. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/4.0
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
Identifier
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000462859300010&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
Subjects
Science & Technology
Life Sciences & Biomedicine
Developmental Biology
Differentiation
Embryonic stem cells
Pluripotency
Self-renewal
GROUND-STATE
NAIVE PLURIPOTENCY
SELF-RENEWAL
GERM-CELLS
IN-VITRO
GENOME
DIFFERENTIATION
DEMETHYLATION
DERIVATION
INHIBITION
Publication Status
Published
Article Number
dev173146
Date Publish Online
2019-03-26