Chronic skin ulcerations are a common complication of diabetes mellitus, affecting up to one in four diabetic individuals. Despite the prevalence of these wounds, current pharmacologic options for treating them remain limited. Growth factor-based therapies have displayed a mixed ability to drive successful healing, which may be due to non-optimal delivery strategies. Here we describe a method for coating commercially available nylon dressings using the Layer-by-Layer (LbL) process to enable both sustained release and independent control over the release kinetics of VEGF and PDGF-BB. We show that the use of strategically spaced diffusion barriers formed spontaneously by disulfide bonds enables independent control over the release rates of incorporated growth factors, and that in vivo these dressings improve several aspects of wound healing in db/db mice.