Manufacture of multilayered artificial cell membranes through sequential bilayer deposition on emulsion templates.
Author(s)
Ip, T
Li, Q
Brooks, N
Elani, Y
Type
Journal Article
Abstract
Efforts to manufacture artificial cells that replicate the architectures, processes and behaviours of biological cells are rapidly increasing. Perhaps the most commonly reconstructed cellular structure is the membrane, through the use of unilamellar vesicles as models. However, many cellular membranes, including bacterial double membranes, nuclear envelopes, and organelle membranes, are multilamellar. Due to a lack of technologies available for their controlled construction, multilayered membranes are not part of the repertoire of cell-mimetic motifs used in bottom-up synthetic biology. To address this, we developed emulsion-based technologies that allow cell-sized multilayered vesicles to be produced layer-by-layer, with compositional control over each layer, thus enabling studies that would otherwise remain inaccessible. We discovered that bending rigidities scale with the number of layers and demonstrate inter-bilayer registration between coexisting liquid-liquid domains. These technologies will contribute to the exploitation of multilayered membrane structures, paving the way for incorporating protein complexes that span multiple bilayers.
Date Issued
2021-07-01
Date Acceptance
2021-02-01
ISSN
1439-4227
Publisher
Wiley-VCH Verlag
Start Page
2275
End Page
2281
Journal / Book Title
ChemBioChem: a European journal of chemical biology
Volume
22
Issue
13
Copyright Statement
© 2021 The Authors. ChemBioChem published by Wiley-VCH GmbH
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Sponsor
Medical Research Council (MRC)
Identifier
https://chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/cbic.202100072
https://www.ncbi.nlm.nih.gov/pubmed/33617681
Grant Number
MR/S031537/1
Subjects
artificial cells
droplet technologies
membrane biophysics
molecular bioengineering
vesicles
artificial cells
droplet technologies
membrane biophysics
molecular bioengineering
vesicles
0304 Medicinal and Biomolecular Chemistry
0601 Biochemistry and Cell Biology
Organic Chemistry
Publication Status
Published
Coverage Spatial
Germany
OA Location
https://chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/cbic.202100072
Date Publish Online
2021-02-22