Enzymatically cross-linked gelatin/chitosan hydrogels: tuning gel properties and cellular response
File(s)daSilvaM-MacromolBiosci-2014-accver.docx (675.12 KB)
Accepted version
Author(s)
Type
Journal Article
Abstract
This work investigates the effect of combining physical and chemical gelation processes in a biopolymer blend: chitosan and tilapia fish gelatin. Chemical (C) gels are obtained by cross-linking with the microbial enzyme transglutaminase at 37 °C. Hybrid physical-co-chemical (PC) gels are cross-linked at 21 °C, below gelatin gelation temperature. These protocols provide two microenvironments for the gelation process: in C gels, both gelatin and chitosan are present as single strands; in PC gels, cross-linking proceeds within a transient physical gel of gelatin, filled by chitosan strands. The chitosan/gelatin chemical networks generated in PC gels show a consistently higher shear modulus than pure C gels; they are also less turbid than their C gels counterparts, suggesting a more homogeneous network. Finally, chitosan enhances the gels' shear modulus in all gels. Proliferation assays show that MC3T3 cells proliferate in these mixed, hybrid gels and better so on PC gels than in C mixed gels
Date Issued
2014-02-18
Date Acceptance
2014-02-01
ISSN
1616-5187
Publisher
Wiley
Start Page
817
End Page
830
Journal / Book Title
Macromolecular Bioscience
Volume
14
Issue
6
Copyright Statement
© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. This is the accepted version of the following article: da Silva, M. A., Bode, F., Drake, A. F., Goldoni, S., Stevens, M. M. and Dreiss, C. A. (2014), Enzymatically Cross-Linked Gelatin/Chitosan Hydrogels: Tuning Gel Properties and Cellular Response. Macromol. Biosci., 14: 817–830, which has been published in final form at https://dx.doi.org/10.1002/mabi.201300472
Subjects
Science & Technology
Life Sciences & Biomedicine
Technology
Physical Sciences
Biochemistry & Molecular Biology
Materials Science, Biomaterials
Polymer Science
Materials Science
chitosan
enzymatic cross-linking
hydrogels
rheology
tilapia fish gelatin
VISCOELASTIC PROPERTIES
CHEMICAL NETWORKS
GELATIN NETWORKS
CHITOSAN
TRANSGLUTAMINASE
CONFORMATIONS
BIOMATERIALS
STABILITY
PEPTIDES
COLLAGEN
Animals
Cell Line
Cell Proliferation
Chitosan
Gelatin
Hydrogels
Materials Testing
Mice
Osteoblasts
Transglutaminases
0303 Macromolecular And Materials Chemistry
0903 Biomedical Engineering
Polymers
Publication Status
Published