Expanding and optimizing 3D bioprinting capabilities using complementary network bioinks
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Published version
Author(s)
Type
Journal Article
Abstract
A major challenge in 3D bioprinting is the limited number of bioinks that fulfill the physiochemical requirements of printing, while also providing a desirable environment for encapsulated cells.Here, we address this limitation by temporarily stabilizing bioinks with a complementary thermo-reversible gelatin network. This strategy enablesthe effective printing of biomaterials that would typically not meet printing requirements, with instrument parameters and structural output largely independent of the base biomaterial. This approach is demonstrated across a library of photo-crosslinkable bioinks derived from natural and synthetic polymers, including gelatin, hyaluronic acid, chondroitin sulfate, dextran, alginate, chitosan, heparin,and poly(ethylene glycol). A range of complex and heterogeneous structures are printed, including soft hydrogel constructs supporting the 3D culture of astrocytes. This highly generalizable methodology expands the palette of available bioinks, allowing the biofabrication of constructs optimized to meet the biological requirements of cell culture and tissue engineering.
Date Issued
2020-09-18
Date Acceptance
2020-07-22
Citation
Science Advances, 2020, 6 (38), pp.1-13
ISSN
2375-2548
Publisher
American Association for the Advancement of Science
Start Page
1
End Page
13
Journal / Book Title
Science Advances
Volume
6
Issue
38
Copyright Statement
© 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). https://creativecommons.org/licenses/by-nc/4.0/
This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license, which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.
This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license, which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.
Sponsor
Engineering & Physical Science Research Council (E
Medical Research Council (MRC)
Wellcome Trust
Commission of the European Communities
Medical Research Council (MRC)
Identifier
https://advances.sciencemag.org/content/6/38/eabc5529
Grant Number
20144247
MR/R015651/1
098411/Z/12/Z
839111
MR/S00551X/1
Publication Status
Published
Date Publish Online
2020-09-18