Trichinella spiralis secretes abundant unencapsulated small RNAs with potential effects on host gene expression
File(s)
Published version
Author(s)
Type
Journal Article
Abstract
Many organisms, including parasitic nematodes, secrete small RNAs into the extracellular environment, largely encapsulated within small vesicles. Parasite-secreted material often contains microRNAs (miRNAs), raising the possibility that they might regulate host genes in target cells. Here we characterise secreted RNAs from the parasitic nematode Trichinella spiralis at two different life stages. We show that adult T. spiralis, which inhabit intestinal mucosa, secrete miRNAs within vesicles. Unexpectedly, T. spiralis muscle stage larvae, which live intracellularly within skeletal muscle cells, secrete miRNAs that appear not to be encapsulated. Notably, secreted miRNAs include a homologue of mammalian miRNA-31, which has an important role in muscle development. Our work therefore suggests that RNAs may be secreted without encapsulation in vesicles, with implications for the biology of T. spiralis infection.
Date Issued
2020-08-01
Date Acceptance
2020-05-28
Citation
International Journal for Parasitology, 2020, 50 (9), pp.697-705
ISSN
0020-7519
Publisher
Elsevier
Start Page
697
End Page
705
Journal / Book Title
International Journal for Parasitology
Volume
50
Issue
9
Copyright Statement
© 2020 The Authors. Published by Elsevier Ltd on behalf of Australian Society for Parasitology.This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/)
License URL
Sponsor
Biotechnology and Biological Sciences Research Council (BBSRC)
Identifier
https://www.ncbi.nlm.nih.gov/pubmed/32622688
PII: S0020-7519(20)30159-4
Grant Number
BB/L023091/1
Subjects
Extracellular vesicles
Host-parasite interactions
MicroRNA
Trichinella spiralis
Publication Status
Published
Coverage Spatial
England
Date Publish Online
2020-07-03