The Nrd1-like protein Seb1 coordinates cotranscriptional 3′ end processing and polyadenylation site selection
File(s)
Accepted version
Author(s)
Type
Journal Article
Abstract
Termination of RNA polymerase II (RNAPII) transcription is associated with RNA 3′ end formation. For coding genes, termination is initiated by the cleavage/polyadenylation machinery. In contrast, a majority of noncoding transcription events in Saccharomyces cerevisiae does not rely on RNA cleavage for termination but instead terminates via a pathway that requires the Nrd1–Nab3–Sen1 (NNS) complex. Here we show that the Schizosaccharomyces pombe ortholog of Nrd1, Seb1, does not function in NNS-like termination but promotes polyadenylation site selection of coding and noncoding genes. We found that Seb1 associates with 3′ end processing factors, is enriched at the 3′ end of genes, and binds RNA motifs downstream from cleavage sites. Importantly, a deficiency in Seb1 resulted in widespread changes in 3′ untranslated region (UTR) length as a consequence of increased alternative polyadenylation. Given that Seb1 levels affected the recruitment of conserved 3′ end processing factors, our findings indicate that the conserved RNA-binding protein Seb1 cotranscriptionally controls alternative polyadenylation.
Date Issued
2016-07-01
Date Acceptance
2016-06-10
Citation
Genes & Development, 2016, 30 (13), pp.1558-1572
ISSN
1549-5477
Publisher
Cold Spring Harbor Laboratory Press
Start Page
1558
End Page
1572
Journal / Book Title
Genes & Development
Volume
30
Issue
13
Copyright Statement
© 2016 Lemay et al. Published by Cold Spring Harbor Laboratory Press.
Subjects
3′ end processing
Nrd1
S. pombe
Seb1
polyadenylation
transcription termination
Developmental Biology
Biological Sciences
Medical And Health Sciences
Psychology And Cognitive Sciences
Publication Status
Published