High performance thin-layer chromatography of plant ecdysteroids coupled with desorption electrospray ionisation–ion mobility–time of flight high resolution mass spectrometry (HPTLC/DESI/IM/ToFMS)
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Author(s)
Claude, E
Tower, M
Lafont, R
Wilson, ID
Plumb, RS
Type
Journal Article
Abstract
The use of high performance thin-layer chromatography (HPTLC) in combination with high resolution time of flight mass spectrometry (MS) for the detection, identification and imaging (HPTLC/MSI) of ecdysteroids (insect moulting hormones) present in a number of plant extracts obtained from members of the Silene family is demonstrated. DESI is shown to be a convenient method for the recovery of these polar polyhydroxylated steroids from the silica gel of the HPTLC plate for subsequent MS detection and imaging. The incorporation of an ion mobility separation (IMS) to the system to give HPTLC/IMS/MS provided additional drift time data which enabled more confident identification. Using HPTLC/DESI/IMS/MS, a range of ecdysteroids were detected and characterized in extracts of S. otitis, S nutans, S. maritime, S. viridiflora and S. fimbriata.
Date Issued
2020-08
Date Acceptance
2020-05-29
Citation
Chromatographia, 2020, 83 (8), pp.1029-1035
ISSN
0009-5893
Publisher
Springer Science and Business Media LLC
Start Page
1029
End Page
1035
Journal / Book Title
Chromatographia
Volume
83
Issue
8
Copyright Statement
© The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long
as you give appropriate credit to the original author(s) and the source,
provide a link to the Creative Commons licence, and indicate if changes
were made. The images or other third party material in this article are
included in the article’s Creative Commons licence, unless indicated
otherwise in a credit line to the material. If material is not included in
the article’s Creative Commons licence and your intended use is not
permitted by statutory regulation or exceeds the permitted use, you will
need to obtain permission directly from the copyright holder. To view a
copy of this licence, visit http://creativecommons.org/licenses/by/4.0/
as you give appropriate credit to the original author(s) and the source,
provide a link to the Creative Commons licence, and indicate if changes
were made. The images or other third party material in this article are
included in the article’s Creative Commons licence, unless indicated
otherwise in a credit line to the material. If material is not included in
the article’s Creative Commons licence and your intended use is not
permitted by statutory regulation or exceeds the permitted use, you will
need to obtain permission directly from the copyright holder. To view a
copy of this licence, visit http://creativecommons.org/licenses/by/4.0/
License URL
Identifier
https://link.springer.com/article/10.1007%2Fs10337-020-03917-9
Subjects
Analytical Chemistry
0301 Analytical Chemistry
0399 Other Chemical Sciences
Publication Status
Published
Date Publish Online
2020-06-07