Enhancing precursor supply and engineering efflux systems to improve abscisic acid production and secretion in Yarrowia lipolytica
Author(s)
Type
Journal Article
Abstract
Abscisic acid is a sesquiterpene phytohormone with extensive applications in agriculture and human health. Currently, it is produced through fermentation of Botrytis cinerea, a plant pathogenic filamentous fungus. The process requires morphology controls, which complicates production and strain optimization. In this study, the abscisic acid production strain Yarrowia lipolytica SM309 was optimized by enhancing the precursor supply using a “push–pull–restrain” strategy focusing on acetyl-CoA, which increased abscisic acid production from 266.34 to 328.51 mg/L. Subsequently, in silico prediction and analysis were used to obtain the docking conformations and binding affinity of ABC transporters for abscisic acid. Overexpression of ABC transporter YlGcn20 further enhanced abscisic acid production by 10.88%, reaching 354.21 mg/L. Additionally, low temperature and dodecane addition were employed as auxiliary strategies to promote abscisic acid synthesis, resulting in a titer of 605.92 mg/L. Finally, the engineered strain achieved an abscisic acid titer of 2056.64 mg/L in a 5 L bioreactor, representing the highest titer reported for a yeast de novo synthesis system to date.
Date Issued
2025-03-12
Date Acceptance
2025-02-20
Citation
Journal of Agricultural and Food Chemistry, 2025, 73 (10), pp.6050-6058
ISSN
0021-8561
Publisher
American Chemical Society
Start Page
6050
End Page
6058
Journal / Book Title
Journal of Agricultural and Food Chemistry
Volume
73
Issue
10
Copyright Statement
Copyright © 2025 American Chemical Society. This is the author’s accepted manuscript made available under a CC-BY licence in accordance with Imperial’s Research Publications Open Access policy (www.imperial.ac.uk/oa-policy)
License URL
Identifier
https://www.ncbi.nlm.nih.gov/pubmed/40011064
Subjects
<italic>Yarrowialipolytica</italic>
ABC transporter
ABC TRANSPORTER
abscisic acid
Agriculture
Agriculture, Multidisciplinary
Chemistry
Chemistry, Applied
Food Science & Technology
Life Sciences & Biomedicine
Physical Sciences
precursor supply
PREDICTION
Science & Technology
sesquiterpene
STRATEGIES
Publication Status
Published
Coverage Spatial
United States
Date Publish Online
2025-02-26