Host resistance to pulmonary Mycobacterium tuberculosis 2 infection requires CD153 expression
File(s)
Accepted version
Author(s)
Type
Journal Article
Abstract
Mycobacterium tuberculosis infection (Mtb) is the leading cause of death due to a single infectious agent and is among the top ten causes of all human deaths worldwide1. CD4 T cells are essential for resistance to Mtb infection, and for decades it has been thought that IFNγ production is the primary mechanism of CD4 T-cell-mediated protection2,3. However, IFNγ responses do not correlate with host protection, and several reports demonstrate that additional anti-tuberculosis CD4 T-cell effector functions remain unaccounted for4,5,6,7,8. Here we show that the tumour-necrosis factor (TNF) superfamily molecule CD153 (encoded by the gene Tnfsf8) is required for control of pulmonary Mtb infection by CD4 T cells. In Mtb-infected mice, CD153 expression is highest on Mtb-specific T helper 1 (TH1) cells in the lung tissue parenchyma, but its induction does not require TH1 cell polarization. CD153-deficient mice develop high pulmonary bacterial loads and succumb early to Mtb infection. Reconstitution of T-cell-deficient hosts with either Tnfsf8−/− or Ifng−/− CD4 T cells alone fails to rescue mice from early mortality, but reconstitution with a mixture of Tnfsf8−/− and Ifng−/− CD4 T cells provides similar protection as wild-type T cells. In Mtb-infected non-human primates, CD153 expression is much higher on Ag-specific CD4 T cells in the airways compared to blood, and the frequency of Mtb-specific CD153-expressing CD4 T cells inversely correlates with bacterial loads in granulomas. In Mtb-infected humans, CD153 defines a subset of highly polyfunctional Mtb-specific CD4 T cells that are much more abundant in individuals with controlled latent Mtb infection compared to those with active tuberculosis. In all three species, Mtb-specific CD8 T cells did not upregulate CD153 following peptide stimulation. Thus, CD153 is a major immune mediator of host protection against pulmonary Mtb infection and CD4 T cells are one important source of this molecule.
Date Issued
2018-09-10
Date Acceptance
2018-07-27
Citation
Nature Microbiology, 2018, 3, pp.1198-1205
ISSN
2058-5276
Publisher
Nature Publishing Group
Start Page
1198
End Page
1205
Journal / Book Title
Nature Microbiology
Volume
3
Copyright Statement
© 2018 Springer Nature Limited. All rights reserved.
Sponsor
Wellcome Trust
Grant Number
104803/Z/14/ZR
Subjects
Science & Technology
Life Sciences & Biomedicine
Microbiology
T-CELL RESPONSES
INTERFERON-GAMMA
PROTECTIVE IMMUNITY
DISEASE
TH1
PHENOTYPE
VACCINE
HUMANS
Publication Status
Published